Effect of solvent on chlorophyll extraction of fresh and dried Gracilaria sp. and Caulerpa sp. seaweed

Authors

DOI:

https://doi.org/10.36733/medicamento.v9i1.5344

Keywords:

chlorophyll, drying, seaweed, solvent extraction, chlorophyll, drying, seaweed, solvent extraction

Abstract

Seaweed is a photosynthetic organism, thus a good source of pigment-producing. Pharmaceutical, food, and cosmetic industries widely use chlorophyll pigments for their products. Bulung Sangu (Gracilaria sp.) and Bulung Boni (Caulerpa sp.) are widely grown seaweeds in the waters of Bali. Pre-extraction steps such as drying and solvent selection can affect pigment extraction. This study aimed to evaluate the effect of solvent selection in the extraction of chlorophyll in fresh and dried Bulung Sangu (Gracilaria sp.) and Bulung Boni (Caulerpa sp.). The solvents used were ethanol, methanol, and acetone. Estimation of chlorophyll content was carried out using UV-VIS spectrophotometry. Data were analyzed statistically using one-way ANOVA with a 95% confidence level. The results showed that the extraction of Bulung Sangu (Gracilaria sp.) on dry samples using acetone produced a total chlorophyll of 574.1 ± 33.2 g/g. Bulung Boni (Caulerpa sp.) extraction of fresh samples using methanol resulted in the highest total chlorophyll (10235.3 ± 50.3 g/g). In conclusion, drying and solvent selection affect the extraction of chlorophyll from Bulung Sangu (Gracilaria sp.) and Bulung Boni (Caulerpa sp.).

Author Biographies

Maria Malida Vernandes Sasadara, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Ni Made Dwi Mara Widyani Nayaka, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Putu Era Sandhi Kusuma Yuda, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Erna Cahyaningsih, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Ni Luh Kade Arman Anita Dewi, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

References

Guiry MD. What are algae? The Seaweed Site: information on marine algae. Published 2022. https://www.seaweed.ie/algae/algae.php

Khan MI, Shin JH, Kim JD. The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products. Microb Cell Fact. 2018;17(1):1-21. doi:10.1186/s12934-018-0879-x

de Almeida CLF, Falcão H de S, Lima GR d. M, et al. Bioactivities from marine algae of the genus Gracilaria. Int J Mol Sci. 2011;12(7):4550-4573. doi:10.3390/ijms12074550

Sasadara MMV, Wirawan IGP, Jawi IM, Sritamin M, Dewi NNA, Adi AAAM. Anti-inflammatory Effect of Red Macroalgae Bulung Sangu (Gracilaria sp.) Extract in UVB-Irradiated Mice. Pakistan J Biol Sci. Published online 2020. doi:10.3923/pjbs.2021.80.89

Mukherjee PK, Maity N, Nema NK, Sarkar BK. Bioactive compounds from natural resources against skin aging. Phytomedicine. 2011;19(1):64-73. doi:10.1016/j.phymed.2011.10.003

Turkmen N, Sari F, Velioglu YS. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem. 2006;99(4):835-841. doi:10.1016/j.foodchem.2005.08.034

Do QD, Angkawijaya AE, Tran-Nguyen PL, et al. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal. 2014;22(3):296-302. doi:10.1016/j.jfda.2013.11.001

Sasadara MMV, Wirawan IGP. Effect of extraction solvent on total phenolic content, total flavonoid content, and antioxidant activity of Bulung Sangu (Gracilaria sp.) Seaweed. IOP Conf Ser Earth Environ Sci. 2021;712(1). doi:10.1088/1755-1315/712/1/012005

Monteiro M, Santos RA, Iglesias P, et al. Effect of extraction method and solvent system on the phenolic content and antioxidant activity of selected macro- and microalgae extracts. J Appl Phycol. 2020;32(1):349-362. doi:10.1007/s10811-019-01927-1

Krakowska-Sieprawska A, Kiełbasa A, Rafińska K, Ligor M, Buszewski B. Modern Methods of Pre-Treatment of Plant Material for the Extraction of Bioactive Compounds. Molecules. 2022;27(3). doi:10.3390/molecules27030730

Hosikian A, Lim S, Halim R, Danquah MK. Chlorophyll extraction from microalgae: A review on the process engineering aspects. Int J Chem Eng. 2010;2010. doi:10.1155/2010/391632

Sasadara M, Nayaka N, Yuda P, et al. Optimization of chlorophyll extraction solvent of bulung sangu (Gracilaria sp.) seaweed. IOP Conf Ser Earth Environ Sci. 2021;913(1). doi:10.1088/1755-1315/913/1/012073

Osório C, Machado S, Peixoto J, et al. Pigments Content (Chlorophylls, Fucoxanthin and Phycobiliproteins) of Different Commercial Dried Algae. Separations. 2020;7(2):33. doi:10.3390/separations7020033

Doli R, Mantiri DM, Paransa D, Kremer K, Lintang R, Tumembow S. Analisis pigmen pada alga Kappaphycus alvarezii yang dibudidayakan di teluk Totok Kabupaten Minahasa Tenggara. J Pesisir Dan Laut Trop. 2020;8(1):31. doi:10.35800/jplt.8.1.2020.27399

Verdiana M, Widarta IWR, Permana IDGM. Pengaruh Jenis Pelarut Pada Ekstraksi Menggunakan Gelombang Ultrasonik Terhadap Aktivitas Antioksidan Ekstrak Kulit Buah Lemon. J Ilmu dan Teknol Pangan. 2018;7(4):213. doi:10.24843/itepa.2018.v07.i04.p08

Onyebuchi C, Kavaz D. Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts. Sci Rep. 2020;10(1):1-11. doi:10.1038/s41598-020-78847-5

Brás T, Neves LA, Crespo JG, Duarte MF. Effect of extraction methodologies and solvent selection upon cynaropicrin extraction from Cynara cardunculus leaves. Sep Purif Technol. 2020;236:116283. doi:10.1016/j.seppur.2019.116283

Connan S. Spectrophotometric Assays of Major Compounds Extracted from Algae. In: Stengel DB, Connan S, eds. Natural Products From Marine Algae: Methods and Protocols, Methods in Molecular Biology. Vol 1308. Springer Science+Business Media New; 2015:1-439. doi:10.1007/978-1-4939-2684-8

Ritchie RJ. Universal chlorophyll equations for estimating chlorophylls a, b, c, and d and total chlorophylls in natural assemblages of photosynthetic organisms using acetone, methanol, or ethanol solvents. Photosynthetica. 2008;46(1):115-126. doi:10.1007/s11099-008-0019-7

Syad AN, Shunmugiah KP, Kasi PD. Seaweeds as nutritional supplements: Analysis of nutritional profile, physicochemical properties and proximate composition of G.Acerosa and S.Wightii. Biomed Prev Nutr. 2013;3(2):139-144. doi:10.1016/j.bionut.2012.12.002

Saini RK, Keum YS. Carotenoid extraction methods: A review of recent developments. Food Chem. 2018;240(June 2017):90-103. doi:10.1016/j.foodchem.2017.07.099

Kha TC, Nguyen MH, Roach PD, Stathopoulos CE. Effect of drying pre-treatments on the yield and bioactive content of oil extracted from Gac Aril. Int J Food Eng. 2013;10(1):103-112. doi:10.1515/ijfe-2013-0028

Sari IN. Perbandingan Kadar Flavonoid Total Seduhan Daun Segar dan Daun Kering Benalu Cengkeh (Dendrophthoe petandra L.) dengan Metode Spektrofotometri Uv-Vis. Published online 2020. http://librepo.stikesnas.ac.id/393/

Negi PS, Roy SK. Effect of Blanching and Drying Methods on β -Carotene, Ascorbic acid and Chlorophyll Retention of Leafy Vegetables. Lwt. 2000;33(4):295-298. doi:10.1006/fstl.2000.0659

Patel BH. Natural Dyes. Vol 1. Woodhead Publishing Limited; 2011. doi:10.1533/9780857093974.2.395

Pareek S, Sagar NA, Sharma S, et al. Chlorophylls: Chemistry and biological functions. Fruit Veg Phytochem Chem Hum Heal Second Ed. 2017;1(May):269-284. doi:10.1002/9781119158042.ch14

Walsh E. Types of Chlorophyll Present in Algae. Sciencing. Published 2019. https://sciencing.com/types-chlorophyll-present-algae-8433014.html

Dasgupta CN. Algae as a source of phycocyanin and other industrially important pigments. In: Das D, ed. Algae Biorefinery: An Integrated Approach, 1st Ed. Springer International Publishing; 2015.

Brereton RG, Rahmani A, Liang Y ‐z, Kvalheim OM. Investigation of the allomerization reaction of chlorophyll a: use of diode array HPLC, mass spectrometry and chemometric factor analysis for the detection of early products. Photochem Photobiol. 1994;59(1):99-110. doi:10.1111/j.1751-1097.1994.tb05007.x

Pigmen Klorofil ekstraksi dengan beberapa pelarut

Published

2023-03-30

How to Cite

Sasadara, M. M. V., Nayaka, N. M. D. M. W., Yuda, P. E. S. K., Cahyaningsih, E., & Dewi, N. L. K. A. A. (2023). Effect of solvent on chlorophyll extraction of fresh and dried Gracilaria sp. and Caulerpa sp. seaweed. Jurnal Ilmiah Medicamento, 9(1), 22–28. https://doi.org/10.36733/medicamento.v9i1.5344

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