Phytochemical Screening and Antioxidant Activity of Orange Mistletoe (Dendrophthoe glabrescens (Blakely) Barlow) Leaf Extracts with n-Hexan and Ethanol Solvents
DOI:
https://doi.org/10.36733/medicamento.v9i2.7136Keywords:
Antioxidant, Leaves of Orange Mistletoe, ScreeningAbstract
One of the compounds that can be exploited from plants is antioxidants. Antioxidants are compounds that are important in overcoming free radicals in the body. Natural antioxidants are more desirable than synthetic ones because of their minimal side effects. Mistletoe is a parasitic plant that has been used in traditional medicine in Indonesia. This study aims to determine the content of secondary metabolites contained in citrus parasite leaf extracts using non-polar and polar solvents and how their antioxidant activity is. with a ratio of material and solvent 1:10. The screening tested included alkaloids, triterpenoids, steroids, flavonoids, saponins, and tannins. The method used to test the antioxidant activity of the orange parasite leaf extract is the DPPH method as measured by a UV-Vis spectrophotometer. Statistical analysis was carried out using One Way Anova and Post Hoc on the IC50 value of the extract with a 95% level of confidence. From the results of research conducted, the N-Hexane extract of citrus parasite leaves contained secondary metabolites of alkaloids, steroids and flavonoids, and the ethanol extract of orange parasite leaves contained secondary metabolites of alkaloids, steroids, flavonoids, saponins, and tannins. The antioxidant activity of the n-Hexane and ethanol extracts were 92.731 ppm (strong) and 54.490 ppm (strong) respectively.
References
Yulian M, Safrijal S. Uji Aktivitas Antioksidan Daun Benalu Kopi (Loranthus Ferrugineus Roxb.) dengan Metode DPPH (1,1 – Difenil -2- Pikrilhidrazil). Lantanida J. 2019;6(2):192. doi:10.22373/lj.v6i2.4127
Selawati R. Penapisan Fitokimia Berbagai Benalu Yang Digunakan Sebagai Obat di Desa Sumberjaya Kecamatan Waway Karya Lampung TImur. 2019;2.
Sembiring HB, Lenny S, Marpaung L. Aktivitas Antioksidan Senyawa Flavonoida dari Daun Benalu Kakao (Dendrophthoe pentandra (L.) Miq.). Chim Nat Acta. 2016;4(3):117. doi:10.24198/cna.v4.n3.10920
Arnanda QP, Nuwarda RF. Penggunaan Radiofarmaka Teknisium-99M Dari Senyawa Glutation dan Senyawa Flavonoid Sebagai Deteksi Dini Radikal Bebas Pemicu Kanker. Farmaka Suplemen. 2019;14(1):1-15.
Sepriyani et. a. Aktivitas Antioksidan Ekstrak Etanol Daun Pepaya (Carica papaya L) dengan Metode 2,2-Diphenyl -1-Picrylhydrazil (DPPH). J Penelit Farm Indones. 2020;9(1):8-11.
Rahayu S, Kurniasih N, Amalia V. Ekstraksi Dan Identifikasi Senyawa Flavonoid Dari Limbah Kulit Bawang Merah Sebagai Antioksidan Alami. al-Kimiya. 2015;2(1):1-8. doi:10.15575/ak.v2i1.345
Ndruru MF, Kosasih E. Uji efektivitas ekstrak daun benalu jeruk (Dendrophthoe glabrescens) sebagai nefroprotektor terhadap kerusakan ginjal yang diinduksi dengan paracetamol pada tikus putih (Rattus novergicus). J Biosains. 2019;5(2):45-52. doi:https://doi.org/10.24114/jbio.v5i2.12634 ISSN
Nwoke SC, Imaga NOA, Omojufehinsi M, Magbagbeola AO, Ebuehi OAT, Okafor UA. Nutritional and Phytochemical Properties of Aqueous Leaf Extract of Mistletoe (Tapinanthus Bangwensis) Grown on Orange Tree. Univ Lagos J Basic Med Sci. 2022;10(1-2):35-41. doi:10.52968/23681529
Faiqoh Z, Baskara AANN, Budi DS, Putra PP, Nitari W, Solikhah EN. Uji Aktivitas Antiplasmodium Ekstrak Benalu Secara in Vivo Pada Mencit Galur Swiss. e-Proceedings PIMNAS PKM-P. 2017;2(1):1-4.
Slamet S, Laula L, Khanifah M. Uji Toksisitas Fraksi N-Heksan dan Etanol, Ekstrak Daun Dendrophthoe glabrescen (Benalu Jeruk) sebagai Skrining Awal Anti-Kanker dengan Metode Brine Shrimp Lethality Test (BSLT). URECOL. Published online 2020:52-57.
Handayani V, Ahmad AR, Sudir M. Uji Aktivitas Antioksidan Ekstrak Metanol Bunga dan Daun Patikala ( Etlingera elatior ( Jack ) R . M . Sm ) Menggunakan Abstrak. Pharm Sci Res. 2014;1(2):86-93.
Tamunu MSS, Pareta DN, Hariyadi, A.Karauwan F. Skrining Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Daun Benalu Pada Kersen Dendropthoe Petandra (L.) Dengan Metode 2,2- Diphenyl -1- Picryhydrazyl (DPPH). Trop J Biopharm. 2022;5(1):79-82.
Huliselan YM, Runtuwene MRJ, Wewengkang DS. Aktivitas Antioksidan Ekstrak Etanol, Etil Asetat, dan n-Heksan dari Daun Sesewanua (Clerodendron squamatum Vahl.). Pharmacon. 2015;4(3):155-163.
Klau MHC, Hesturini RJ. Pengaruh Pemberian Ekstrak Etanol Daun Dandang Gendis (Clinacanthus nutans (Burm F) Lindau) Terhadap Daya Analgetik Dan Gambaran Makroskopis Lambung Mencit. J Farm Sains Indones. 2021;4(1):6-12. doi:10.52216/jfsi.v4i1.59
BRIN. Hasil Determinasi Tanaman “Dendrophtoe Glabrescens (Blakely) Barlow.” Badan Ris Inov Nas. Published online 2023.
Wahyuni IR. Validasi Metode Analisis Uji Aktivitas Antioksidan Ekstrak NHeksan, Etil Asetat, Etanol 70% Umbi Talas Ungu (Colocasia esculenta L. Schott) Dengan Metode DPPH, CUPRAC, dan FRAP secara Spektrofotometri UV-VIS. Published online 2015.
Prasetyo, E. et. a. Uji Aktivitas Antioksidan Menggunakan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Terhadap Ekstrak Etanol Kulit Buah Durian (Durio zibethinnus L.) dari Desa Alasmalang Kabupaten Banyumas. J Pharmascienc. 2021;8(1):75-82.
Irianti T, Sugiyanto, Nuranto S, Kuswandi K. Antioksidan. Universitas Gajah Mada; 2017.
Parwata MOA. Antioksidan. Universitas Udayana; 2016.
Omojufehinsi M, Nwoke SC, Imaga NA, Magbagdeola AO, Ebuehi AO, Okafor UA. Preliminary Investigation of The Nutritional Qualities, Phytochemical Properties and Antioxidant Activities of Aqueous Extract of Mistletoe Leaves (Tapinanthus Bangwensis) Grown on Orange Trees. FASEB J. 2013;27(S1). doi:10.1096/fasebj.27.1_supplement.794.16
Sembiring HB. Isolasi dan Penentuan Struktur Kimia Senyawa Flavonoida Daun Benalu Jeruk (Scurrula fusca G. Don) Serta Uji Aktivitas Antioksidan. Disertasi Dr. Published online 2016.
Hartati D. Uji Aktivitas Antioksidan Ekstrak Etanol dan Fraksi Daun Benalu (Scurrula atropurpurea (Bl.) Denser) yang Tumbuh pada Inang Rambutan dengan Metode DPPH. Skripsi. Published online 2016.
Purwanto, D. et. a. Uji Aktivitas Purnajiwa (Kopsia arborea blume) Dengan Berbagai Pelarut. J Kovalen. 2017;3(1):24 – 32.
Udayani NNW, Ratnasari NLAM, Nida IDAAY. Penetapan Kadar Senyawa Fitokimia (Alkaloid, Flavonoid dan Tanin) pada Ekstrak Etanol Rimpang Kunyit Hitam (Curcuma Caesia Roxb.). J Pendidik Tambusai,. 2022;6(1):2088–2093. https://jptam.org/index.php/jptam/article/view/3256
Fajarwati N. Uji Aktivitas Antioksidan pada Ekstrak Daun Jeruk Nipis (Citrus aurantifolia) dengan Menggunakan Metode DPPH. Skripsi. Published online 2013.
Anggreni NPPC, Yanti NPRD, Pratiwi KAP, Udayani NNW. Uji Aktivitas Antioksidan Gummy Candy Ekstrak Daun Sirih Cina (Peperomia pellucida L. Kunth) dengan Metode DPPH. Indones J Pharm Educ. 2023;3(3):436-446. https://ejurnal.ung.ac.id/index.php/ijpe/article/view/22117
Monikasari M, Widyastiti NS, Mahati E, Syauqy A, Al-Baarri AN. Pengaruh pemberian ekstrak bekatul beras hitam (Oryza sativa L. indica) terhadap kadar MDA, SOD dan trigliserida pada tikus diabetes mellitus tipe 2. AcTion Aceh Nutr J. 2023;8(1):129. doi:10.30867/action.v8i1.731
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