Total Flavonoid Content and Antioxidant Activity of Different Polarity Extracts from Pereskia bleo Leaves


  • Ni Made Dwi Mara Widyani Nayaka Universitas Mahasaraswati Denpasar
  • Erna Cahyaningsih Universitas Mahasaraswati Denpasar
  • Maria Malida Vernandes Sasadara Universitas Mahasaraswati Denpasar
  • Putu Era Sandhi Kusuma Yuda Universitas Mahasaraswati Denpasar
  • Felia Riska Indriani Universitas Mahasaraswati Denpasar



Antioxidant, DPPH, total flavonoid content, Pereskia bleo


Antioxidant agents are essential for the body due to its ability to scavenge free radicals. Medicinal plants contain phytochemicals that act as antioxidants. The current research aimed to determine the total flavonoid content (TFC) and antioxidant activity of Pereskia bleo leaves extracts from various solvents with different polarities. The nonpolar solvent (n-hexane) was used as the first step of extraction and its residues were then macerated using semi-polar (ethyl acetate) and polar (ethanol 96%) solvents consecutively. The TFC was determined using the colorimetric method while antioxidant activity was examined through the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Antioxidant activity was presented as Inhibitory Concentration 50 (IC50) and Antioxidant Activity Index (AAI). It is noticeable that among analyzed extracts, the ethyl acetate extract of P. bleo leaves contained the highest flavonoid content (15.052 ± 0.172 g quercetin equivalent/100 g extract). Furthermore, the greatest antioxidant activity was obtained from n-hexane extract with the value of IC50 and AAI being 217.307 ppm and 0.230, respectively. Pearson coefficient correlation (r) between TFC and AAI was -0.106. The current study concluded that P. bleo leaves extracts using solvents with different polarities showed variation in TFC values and antioxidant activity. Moreover, TFC was not the main contributor to the antioxidant activity of P. bleo leaves extracts.

Author Biographies

Ni Made Dwi Mara Widyani Nayaka, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Erna Cahyaningsih, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Maria Malida Vernandes Sasadara, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Putu Era Sandhi Kusuma Yuda, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Felia Riska Indriani, Universitas Mahasaraswati Denpasar

Faculty of Pharmacy


Zareisedehizadeh S, Tan CH, Koh HL. A review of botanical characteristics, traditional usage, chemical components, pharmacological activities, and safety of Pereskia bleo (Kunth) DC. Evidence-based Complement Altern Med. 2014;2014. doi:10.1155/2014/326107

Nayaka NMDMW, Fidrianny I, Sukrasno, Hartati R, Singgih M. Antioxidant and antibacterial activities of multiflora honey extracts from the Indonesian Apis cerana bee. J Taibah Univ Med Sci. 2020;15(3):211-217. doi:10.1016/j.jtumed.2020.04.005

Liu J-Z, Zhang C-C, Fu Y-J, Cui Q. Comparative analysis of phytochemical profile, antioxidant and anti-inflammatory activity from Hibiscus manihot L. flower. Arab J Chem. 2022;15(1):103503. doi:10.1016/J.ARABJC.2021.103503

Sim KS, Sri Nurestri AM, Norhanom AW. Phenolic content and antioxidant activity of Pereskia grandifolia Haw. (Cactaceae) extracts. Pharmacogn Mag. 2010;6(23):248-254. doi:10.4103/0973-1296.66945

Behnaz Hassanbaglou. Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae). J Med Plants Res. 2012;6(15):2932-2937. doi:10.5897/jmpr11.760

Wahab SIA, Abdul AB, Mohan SM, Al-Zubairi AS, Elhassan MM, Ibrahim MY. Biological activities of Pereskia bleo extracts. Int J Pharmacol. 2009;5(1):71-75. doi:10.3923/ijp.2009.71.75

Johari MA, Khong HY. Total phenolic content and antioxidant and antibacterial activities of Pereskia bleo. Adv Pharmacol Sci. 2019;2019:1-4. doi:10.1155/2019/7428593

Bakhari NA, Abdullah AR, Osman H, Nordin NH. The relationship between phenolic, tannin and flavonoid content with the antioxidant activity of Pereskia bleo (Kunth). CSSR 2010 - 2010 Int Conf Sci Soc Res. Published online 2010:494-498. doi:10.1109/CSSR.2010.5773828

Agada R, Usman WA, Shehu S, Thagariki D. In vitro and in vivo inhibitory effects of Carica papaya seed on α-amylase and α-glucosidase enzymes. Heliyon. 2020;6(3):e03618. doi:10.1016/j.heliyon.2020.e03618

Chang C-C, Yang M-H, Wen H-M, Chern J. Estimation of Total Flavonoid Content in Propolis by Two Complementary Colorimetric Methods. 2002;10(3):178-182.

Scherer R, Godoy HT. Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chem. 2009;112(3):654-658. doi:10.1016/j.foodchem.2008.06.026

Fidrianny I, Rezeki EN, Ruslan K, Hartati R, Nayaka NMDMW. The waste products of Mangifera indica L. ‘Arumanis’: In vitro antioxidative activities and phytochemical content. Rasayan J Chem. 2021;14(01):374-381. doi:10.31788/RJC.2021.1415907

Thavamoney N, Sivanadian L, Tee LH, Khoo HE, Prasad KN, Kong KW. Extraction and recovery of phytochemical components and antioxidative properties in fruit parts of Dacryodes rostrata influenced by different solvents. J Food Sci Technol. 2018;55(7):2523. doi:10.1007/S13197-018-3170-6

Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006;19(6-7):669-675. doi:10.1016/j.jfca.2006.01.003

Othman A, Mukhtar NJ, Ismail NS, Chang SK, Alam S. Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants. Int Food Res J. 2014;21(2):759-766.

Yan SW, Ramasamy R, Alitheen NBM, Rahmat A. A comparative assessment of nutritional composition, total phenolic, total flavonoid, antioxidant capacity, and antioxidant vitamins of two types of Malaysian underutilized fruits (Averrhoa bilimbi and Averrhoa carambola). Int J Food Prop. 2013;16(6):1231-1244. doi:10.1080/10942912.2011.582975

Shahidi F, Ambigaipalan P. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. J Funct Foods. 2015;18:820-897. doi:10.1016/J.JFF.2015.06.018

Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20(7):933-956. doi:10.1016/0891-5849(95)02227-9




How to Cite

Nayaka, N. M. D. M. W., Cahyaningsih, E. ., Sasadara, M. M. V. ., Yuda, P. E. S. K. ., & Indriani, F. R. . (2023). Total Flavonoid Content and Antioxidant Activity of Different Polarity Extracts from Pereskia bleo Leaves. Jurnal Ilmiah Medicamento, 9(2), 137–141.



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