Antiradical Activity Study of Momordica charantia L Seeds Based on DPPH and its Secondary Metabolites Analysis


  • Baiq Desy Ratnasari STIKES Kusuma Bangsa
  • Diah Miftahul Aini STIKES Kusuma Bangsa
  • Imam Syahputra Yamin STIKES Kusuma Bangsa
  • Gladeva Yugi Antari STIKES Griya Husada Sumbawa



antioxidant, bitter melon seeds, chemical compound, GC-MS


Momordica charantia L seed is classified as the domestic waste even though it has many health benefits, namely as anti-tumor, anti-virus, and as a medicine to increase the body resistance. Therefore, this study was designed to examine the antioxidant activity of the ethanol extract of the seeds using the DPPH method. Furthermore, a GC-MS analysis will be carried out to determine the chemical compounds that play a role in the bioactivity. Based on the absorbance of DPPH in three repetitions, the average value of DPPH inhibition by 95% ethanol extract of bitter melon seeds was 60.45%, in which classified as a good radical absorbance. The GC-MS results showed that the ethanol extract of bitter melon seeds contained ten chemical compounds consisting of sesquiterpenes, fatty acids, and alkanes, which affecting the radical absorbance of M. charantia extract.

Author Biographies

Baiq Desy Ratnasari, STIKES Kusuma Bangsa

Pharmacy Bachelor Program

Diah Miftahul Aini, STIKES Kusuma Bangsa

Pharmacy Bachelor Program

Imam Syahputra Yamin, STIKES Kusuma Bangsa

Hospital Administration Undergraduate Study Program

Gladeva Yugi Antari, STIKES Griya Husada Sumbawa

Midwifery Diploma Three Study Program


Buckle, J. (2015) 'Basic plant taxonomy, basic essential oil chemistry, extraction, biosynthesis, and analysis', Clinical aromatherapy, pp. 37-72.

Güneş, H., Alper, M. and Çelikoğlu, N. (2019) 'Anticancer effect of the fruit and seed extracts of Momordica charantia L.(Cucurbitaceae) on human cancer cell lines', Tropical Journal of Pharmaceutical Research, 18(10), pp. 2057-2065.

Hadi, S., Ratnasari, B. D., SepTiyana, M., Priyambodo, S. and Sudarma, I. M. (2019) 'Antibacterial Assay and Alkaloid Lombine Distribution Study of Voacanga foetida (BI) Rolfe from Lombok Island', Oriental Journal of Chemistry, 35(1), pp. 275.

Hampel, H., Vassar, R., De Strooper, B., Hardy, J., Willem, M., Singh, N., Zhou, J., Yan, R., Vanmechelen, E. and De Vos, A. (2021) 'The β-secretase BACE1 in Alzheimer’s disease', Biological psychiatry, 89(8), pp. 745-756.

Khalid, Z., Hassan, S. M., Mughal, S. S., Hassan, S. K. and Hassan, H. (2021) 'Phenolic Profile and Biological Properties of Momordica charantia', Chemical and Biomolecular Engineering, 6(1), pp. 17.

Maesaroh, K., Kurnia, D. and Al Anshori, J. (2018) 'Perbandingan metode uji aktivitas antioksidan DPPH, FRAP dan FIC terhadap asam askorbat, asam galat dan kuersetin', Chimica et natura acta, 6(2), pp. 93-100.

Mander, L. and Liu, H.-W. (2010) Comprehensive natural products II: chemistry and biology. Elsevier.

Musman, M. 2018. Kimia Organik Bahan Alam. Banda Aceh: Syiah Kuala University Press.

Oktavia, G. A. E., Arifah, F. H., Arifa, N. and Sujarwo, W. (2020) 'Pengetahuan Etnomedisin Masyarakat Bali Tentang Pare (Momordica charantia L.; Cucurbitaceae): Sebuah Kajian Kepustakaan', Buletin Kebun Raya, 23(3).

Ramalingam, R., Palanisamy, S., Mohanraj, A. K., Durisamy, S. and Rajasekaran, N. (2020) 'Chemical Profiling of Momordica charantia L. Seed Essential Oil and Its Antimicrobial Activity', Journal of Essential Oil Bearing Plants, 23(2), pp. 390-396.

Ramdja, A. F., Aulia, R. M. A. and Mulya, P. (2009) 'Ekstraksi kurkumin dari temulawak dengan menggunakan etanol', Jurnal Teknik Kimia, 16(3).

Riferty, F. (2018) 'Uji Aktivitas Antibakteri Ekstrak Dan Fraksi Biji Pare (Momordica Charantia L.) Terhadap Bakteri Propionibacterium Acnes', Jurnal Ilmiah Farmasi Farmasyifa, 1(2), pp. 119-125.

Septiningsih, R., Sutanto, S. and Indriani, D. (2017) 'AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN, BUAH DAN BIJI PARE (Momordica charantina L)', FITOFARMAKA: Jurnal Ilmiah Farmasi, 7(1), pp. 4-1

Subahar, T. S. S. and Lentera, T. (2004) Khasiat & Manfaat Pare. AgroMedia.

Worldmeter (2021) 'Corona Virus: Reported Cases and Deaths by Country or Territory'. Available at: (Accessed 20 Agustus 2021).

Yoshime, L. T., Melo, I. L. P. d., Sattler, J. A. G., Torres, R. P. and Mancini-Filho, J. (2018) 'Bioactive compounds and the antioxidant capacities of seed oils from pomegranate (Punica granatum L.) and bitter gourd (Momordica charantia L.)', Food Science and Technology, 39, pp. 571-580.



How to Cite

Ratnasari, B. D., Aini, D. M., Yamin, I. S., & Antari, G. Y. (2022). Antiradical Activity Study of Momordica charantia L Seeds Based on DPPH and its Secondary Metabolites Analysis. Jurnal Ilmiah Medicamento, 8(1), 56–62.



Original Articles