Effect of solvent on chlorophyll extraction of fresh and dried Gracilaria sp. and Caulerpa sp. seaweed





chlorophyll, drying, seaweed, solvent extraction, chlorophyll, drying, seaweed, solvent extraction


Seaweed is a photosynthetic organism, thus a good source of pigment-producing. Pharmaceutical, food, and cosmetic industries widely use chlorophyll pigments for their products. Bulung Sangu (Gracilaria sp.) and Bulung Boni (Caulerpa sp.) are widely grown seaweeds in the waters of Bali. Pre-extraction steps such as drying and solvent selection can affect pigment extraction. This study aimed to evaluate the effect of solvent selection in the extraction of chlorophyll in fresh and dried Bulung Sangu (Gracilaria sp.) and Bulung Boni (Caulerpa sp.). The solvents used were ethanol, methanol, and acetone. Estimation of chlorophyll content was carried out using UV-VIS spectrophotometry. Data were analyzed statistically using one-way ANOVA with a 95% confidence level. The results showed that the extraction of Bulung Sangu (Gracilaria sp.) on dry samples using acetone produced a total chlorophyll of 574.1 ± 33.2 g/g. Bulung Boni (Caulerpa sp.) extraction of fresh samples using methanol resulted in the highest total chlorophyll (10235.3 ± 50.3 g/g). In conclusion, drying and solvent selection affect the extraction of chlorophyll from Bulung Sangu (Gracilaria sp.) and Bulung Boni (Caulerpa sp.).

Author Biographies

Maria Malida Vernandes Sasadara, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Ni Made Dwi Mara Widyani Nayaka, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Putu Era Sandhi Kusuma Yuda, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Erna Cahyaningsih, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy

Ni Luh Kade Arman Anita Dewi, Universitas Mahasaraswati Denpasar

Department of Natural Medicine, Faculty of Pharmacy


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Pigmen Klorofil ekstraksi dengan beberapa pelarut



How to Cite

Sasadara, M. M. V., Nayaka, N. M. D. M. W., Yuda, P. E. S. K., Cahyaningsih, E., & Dewi, N. L. K. A. A. (2023). Effect of solvent on chlorophyll extraction of fresh and dried Gracilaria sp. and Caulerpa sp. seaweed. Jurnal Ilmiah Medicamento, 9(1), 22–28. https://doi.org/10.36733/medicamento.v9i1.5344



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