Physicochemical Characterization and Dual-Antimicrobial Efficacy of Ananas comosus Peel Extract Nano-Shampoo Against Scalp Pathogens
DOI:
https://doi.org/10.36733/medicamento.v12i2.13819Keywords:
Ananas comosus, Malassezia furfur, nano shampoo, pineapple peel extract, Staphylococcus epidermidisAbstract
Background: Pineapple peel (Ananas comosus (L.) Merr.) is an agricultural waste that contains various secondary metabolites with antimicrobial potential.
Objective: This study aimed to formulate a pineapple peel extract into a nano-shampoo preparation and evaluate its physical quality characteristics and antimicrobial activity against Malassezia furfur and Staphylococcus epidermidis.
Methods: The nano-shampoo was formulated with three different concentrations of extract: 3% (FI), 6% (FII), and 9% (FIII). Physical quality evaluation included organoleptic observations, homogeneity, pH, viscosity, foam height, and storage stability. Antimicrobial activity was tested in vitro using the agar diffusion method and analyzed.
Results: The results showed that all nano-shampoo formulations met the physical quality requirements and exhibited good stability. All formulations demonstrated antimicrobial activity against M. furfur and S. epidermidis, as indicated by the formation of inhibition zones. Statistical analysis showed that the positive control produced significantly larger inhibition zones than all nano-shampoo formulations (p < 0.05), whereas no significant differences were observed among formulations FI, FII, and FIII (p > 0.05). Nevertheless, the 9% extract formulation (FIII) descriptively exhibited the largest inhibition zones among the tested nano-shampoo formulations.
Conclusion: Based on these results, pineapple peel extract nano-shampoo has the potential for development as a natural-based preparation with antimicrobial activity, particularly as a supportive alternative for scalp care.
References
1. Deng L, Ling X, Li L, He Y, Guo M. Advances in Scalp Microbiome Research: Molecular Insights into the Metabolism-Inflammation-Barrier Axis and Dandruff Pathogenesis. Molecules. 2026; 31(12):2093. doi: 10.3390/molecules31122093
2. Han Y, Zhang YJ, Wang HX, Sun YZ, Yang Y, Li ZX, Qi RQ, Gao XH. Malassezia furfur promoting growth of Staphylococcus epidermidis by increasing pH when cultured in a lipid-free environment. Chin Med J (Engl). 2019;132(7):873-876. doi: 10.1097/CM9.0000000000000152
3. Yin CS, Minh Nguyen TT, Yi EJ, Zheng S, Oh S, Yi T. Efficacy of probiotics in hair growth and dandruff control: A systematic review and meta-analysis. Heliyon. 2024;10(9):e29539. doi: 10.1016/j.heliyon.2024.e29539
4. Tynes BE, Johnson CD, Vaish MH, Abbott B, Vučenovic J, Varrassi G, Potharaju P, Lopez Torres Y, Lee Z, Ahmadzadeh S, Shekoohi S, Kaye AD. Ketoconazole Shampoo for Seborrheic Dermatitis of the Scalp: A Narrative Review. Cureus. 2024;16(8):1-8. doi: 10.7759/cureus.67532
5. Mayser P, Genrich F, Meunier L, Nordzieke S. Scalp Microbiome and Dandruff—Exploring Novel Biobased Esters. Cosmetics. 2024;11(5):174. doi: 10.3390/cosmetics11050174
6. Mursidah A, Salam S, Agustina R. Profil Fitokimia dan Uji Aktivitas Antibakteri Ekstrak Kulit Buah Nanas (Ananas comosus (L.) Merr) [Phytochemical Profile and Antibacterial Activity Testing of Pineapple Peel Extract (Ananas comosus (L.) Merr.)]. Proceeding Mulawarman Pharm Conf. 2023;18:1-6. doi: 10.25026/mpc.v18i1.695
7. Aliyah SH, Musfirotun, Antriana N. Aktivitas Antibakteri Isolat Kapang Endofit Dari Kulit Nanas ( Ananas comosus ( L .) Meer ). BIOSENSE. 2021;04(2):20-30. doi: 10.36526/biosense.v4i02.1569
8. Mgeni ST, Emmanuel JK, Mtashobya LA. Potential Contributions of Pineapple Waste to Nutrition, Medicine, Bioenergy Sources, and Environmental Conservation: A Review. Nat Prod Commun. 2025;20(6). doi: 10.1177/1934578X251348402
9. Paz-Arteaga SL, Cadena-Chamorro E, Goméz-García R, Serna-Cock L, Aguilar CN, Torres-León C. Unraveling the Valorization Potential of Pineapple Waste to Obtain Value-Added Products towards a Sustainable Circular Bioeconomy. Sustainability. 2024;16(16):7236. doi: 10.3390/su16167236
10. Soni S, Noor U, Sharma A, Tripathi SM, Sundaram S, Gupta E. Assessment of antimicrobial potential of polyphenol-rich Ananas comosus peel powder at different drying conditions. Indian J Nat Prod Resour [Formerly Nat Prod Radiance (NPR)]. 2025;16(3):417-427. doi: 10.56042/ijnpr.v16i3.15228
11. Octaviani M, Fikrani D, Susanti E. Aktivitas Antijamur Ekstrak Etanol Kulit Buah Ananas comosus (L) Merr. terhadap Trichophyton mentagrophytes dan Malassezia furfur [Antifungal Activity of Ethanolic Pineapple Peel Extract (Ananas comosus (L.) Merr.) against Trichophyton mentagrophytes and Malassezia furfur]. JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X. 2020;12(2):159-165. doi: 10.35617/jfionline.v12i2.35
12. Jacob S, Nair AB. Nanoemulgels as Advanced Topical Drug Delivery Systems: Mechanistic Insights and Therapeutic Applications in Skin Disorders, Infections, Wound Healing, and Cancer. Pharmaceuticals. 2026;19(2):247. doi: 10.3390/ph19020247
13. Jannah RNS, Illiyyin Akib N, Ode Sitti Zubaydah W, Mahmudah A, Septiani Pratiwi I, Anwar I. Review: Pengaruh Polimer Sebagai Stabilizer Terhadap Ukuran Partikel Nanosuspensi (Review: The Effect of Polymer as Stabilizers on Particle Size of Nanosuspensions). Lansau J Ilmu Kefarmasian. 2025;3(1):19-26. doi: 10.33772/lansau.v3i1.40
14. Preeti, Sambhakar S, Malik R, et al. Nanoemulsion: An Emerging Novel Technology for Improving the Bioavailability of Drugs. Bolla PK, ed. Scientifica (Cairo). 2023;2023:1-25. doi: 10.1155/2023/6640103
15. Lubis N, Lubis MS, Yuniarti R, Rani Z. Formulasi dan Evaluasi Sediaan Shampo Nano Ekstrak Bonggol Nanas (ananas comosus (l.) Merr) sebagai Antiketombe [Formulation and Evaluation of Nano Shampoo Preparation from Pineapple Stem Extract (ananas comosus ( l .) Merr) as anti-dandruff]. Mns DAN Kesehat. 2025;8:285-297. doi: 10.31850/makes.v8i1.3509
16. Yan Z, Zhang S, Wu G, et al. Advances in Nanotechnology-Based Topical Delivery Systems for Skincare Applications. Pharmaceutics. Published online 2026:1-18. doi: 10.3390/pharmaceutics18010063
17. Syamsul ES, Anugerah O, Supriningrum R. Penetapan Rendemen Ekstrak Daun Jambu Mawar berdasarkan Variasi Konsentrasi Etanol Dengan Metode Maserasi [Determination Of Mawar Jambu Leaf Extract (Syzygium L. Alston)]. J Ris KEFARMASIAN Indones. 2020;2(3). doi: 10.33759/jrki.v2i3.98
18. Farobbi MI, Pratama IS, Hidayat LH. Studi Farmakognostik Serbuk Jus Kulit Nanas (Ananas comosus (L.) Merr) [Pharmacognostic Study of Pineapple (Ananas comosus (L.) Merr) Peel Juice Powder]. Acta Pharm Indones. 2025;50(1):47-55. doi: 10.5614/api.v50i1.24955
19. Martines-López E, Cárdenas-García D, Cywiak-Córdova D. Measuring Model for the Gravimetric Method by Drying in N-Stages Applied to Materials with High Moisture Content. MAPAN. 2024;39(2):439-443. doi: 10.1007/s12647-023-00709-y
20. Kissinger, Huldani H, Nasrulloh A V. Improving Simplicia of Kerangas Forest by Minimizing Microbial Content Under Ultraviolet Radiation Treatment. Proc Natl Acad Sci India Sect B Biol Sci. 2024;94(1):101-106. doi: 10.1007/s40011-023-01512-0
21. Raharjo SJ, Sari DRT, Maryanty Y, Tresnowati I, Wijayanti ED. Chemical Composition of Active Compounds in Standardized Cinnamon Simplicia (Cinnamomum verum). Maj Obat Tradis. 2025;30(3):315. doi: 10.22146/mot.100943
22. Castro ACL, Lubido ACC, Balderas AKA, Gonzales RKP, Saldo IJP. Phytochemical Analysis and Antibacterial Assessment of Pineapple (Ananas comosus) Peel Extract. South Asian Res J Nat Prod. 2025;8(1):153-161. doi: 10.9734/sarjnp/2025/v8i1188
23. Sari M, Imani CN, Wardani AP. Profil fitokimia eco enzyme berbahan dasar kulit nanas (Ananas comosus) dan daya antibakteri terhadap Lactobacillus acidophilus ATCC 4356: Studi eksperimental [Phytochemical profile of pineapple peel based eco enzyme and antibacterial activity against Lactobacillus Acidophilus ATCC 4356: Study experimental]. Padjadjaran J Dent Res Students. 2026;10(1):118-126. doi: 10.24198/pjdrs.v10i1.69215
24. Tinh NQ, Thanh D Van, Thu N Van, Thi B, Nhung Q, Huyen PN, Hung NP, Thuy NT, Thuy PD, Mi NH, Tam KT. Preparation of nanoemulsions from Elsholtzia kachinensis and Elsholtzia ciliata essential oils via ultrasonic homogenization and their antibacterial and anticancer activities. R Soc Chem. 2025;15:11243-11256. doi: 10.1039/d5ra00386e
25. Sbhatu DB, Berhe GG, Hndeya AG, Abdu A, Mulugeta A, Abraha HB, Weldemichael MY, Tekle HT, Gebru HA, Taye MG, Kidanemariam HG. Hair Washing Formulations from Aloe elegans Todaro Gel: The Potential for Making Hair Shampoo. Adv Pharmacol Pharm Sci. 2020;2020:1-9. doi: 10.1155/2020/8835120
26. Cheni Cheri D, Kizhakke Veettil AS, Pradeep P, Nayak S. Development of EPS‐rich herbal shampoo base fermented using Cyclea peltata leaf powder and Lactobacillus plantarum. J Cosmet Dermatol. 2022;21(10):4999-5009. doi: 10.1111/jocd.14949
27. NDJ-5S / NDJ-8S SERIES DIGITAL DISPLAY VISCOMETER.
28. Mursyida E, Anjeli YP. Nanopartikel Perak dari Ekstrak Kulit Nanas: Potensi Antifungal terhadap Malassezia furfur ATCC 14521 [Silver Nanoparticles Synthesized from Pineapple Peel Extract: Antifungal Potential Against Malassezia furfur ATCC 14521]. J Ilm Ilmu Kesehat dan Kedokt. 2025;3:101-114. doi: 10.55606/termometer.v3i1.4563
29. Abdassah M. Nanopartikel dengan Gelasi Ionik [Nanoparticles Prepared by Ionic Gelation]. Farmaka. 2021;15:45-52. https://jurnal.unpad.ac.id/farmaka/article/view/12138
30. Anwar H, Aminah I, Syamsuriyati. Optimasi dan Uji Aktivitas Nanoemulgel Ekstrak Suruhan (Peperoma pellucida.L) Pada Bakteri MRSA [Optimization and Evaluation of the Antibacterial Activity of Suruhan (Peperomia pellucida L.) Extract Nanoemulgel Against MRSA]. Pharmacol Pharm Sci Journals. 2024;3(2):93-102. doi: 10.51577/papsjournals.v3i2.681
31. Zulbayu LOMA, Juliansyah R, Firawati. Optimasi Konsentrasi Sukrosa Terhadap Transparansi Dan Sifat Fisik Sabun Padat Transparan Minyak Atsiri Sereh Wangi (Cymbopogon citratus L.) [Optimization of Sucrose Concentration on the Transparency and Physical Properties of Transparent Solid Soap Containing Lemongrass Essential Oil (Cymbopogon citratus L.)]. J Mandala Pharmacon Indones. 2020;6(2). doi: 10.35311/jmpi.v6i1.60
32. Noverita Khairani T, Fitri K, Andry M, Eli Sarumaha Pl. Anti-Fungal Activity Testing of Purple Cabbage (Brassica oleracea Linn.) Ethanol Extract in Inhibiting The Fungi Growth Candida albicans and Malassezia furfur. J Biol Trop. 2024;24(4):991-1003. doi: 10.29303/jbt.v24i4.8009
33. Yanthi V, Mahyarudin M, Rialita A. Antifungal Activity of Endophytic Bacteria isolated from Pegagan (Centella asiatica L.) for Inhibition the Growth of Malassezia furfur. J Biol UNAND. 2022;10(1):23. doi: 10.25077/jbioua.10.1.23-32.2022
34. Apriliani PD, Kurniati I, Dermawan A, Indra AIN. Penggunaan Tepung Kacang Kedelai Hitam (Glycine soja L. Merr) Sebagai Media Alternatif Nutrient Agar (NA) Untuk Pertumbuhan Staphylococcus aureus [The Use of Black Soybean (Glycine soja) Flour as an Alternative Nutrient Agar (NA) Medium for the Growth of Staphylococcus aureus]. J Kesehat Siliwangi. 2023;4(1):268-276. doi: 10.34011/jks.v4i1.1490
35. Aviany HB, Pujiyanto S. Analisis Efektivitas Probiotik di dalam Produk Kecantikan sebagai Antibakteri terhadap Bakteri Staphylococcus epidermidis [Evaluation of the Antibacterial Efficacy of Probiotics in Cosmetic Products Against Staphylococcus epidermidis]. Berk Bioteknol. 2020;3(2). https://ejournal2.undip.ac.id/index.php/bb/article/view/9657
36. Rhimi W, Inyang Aneke C, Mosca A, Otranto D, Cafarchia C. In Vitro Azole and Amphotericin B Susceptibilities of Malassezia furfur from Bloodstream Infections Using E-Test and CLSI Broth Microdilution Methods. Antibiotics. 2020; 9(6):361. doi: 10.3390/antibiotics9060361
37. Neli A, Fitriyani F. Formulasi , Uji Sifat Fisik , Uji Stabilitas , dan Uji Aktivitas Antioksidan Gel Ekstrak Kulit Nanas (Ananas Comosus (L) Merr.) [Formulation, Physical Property Evaluation, Stability Testing, and Antioxidant Activity Assessment of Pineapple Peel (Ananas comosus (L.) Merr.) Extract Gel]. LUMBUNG Farm. 2025;6(1):1-6. doi: 10.31764/lf.v6i1.21142
38. Baitariza A, Ahmad Ghazali R. Formulasi Larutan Obat Kumur Pencegah Plak Gigi Ekstrak Kulit Nanas (Ananas comosus L. Merr) [Formulation of an Anti-Dental Plaque Mouthwash Containing Pineapple Peel (Ananas comosus (L.) Merr.) Extract]. J Sabdariffarma. 2020;6(1):33-42. doi: 10.53675/jsfar.v2i1.29
39. Sulhatun S, Juliati E, Sylvia N, Jalaluddin J, Bahri S. Formulasi Pembuatan Shampo dengan Bahan Baku Minyak Kemiri (Aleurites Moluccana) untuk Kesehatan Rambut [Formulation of a Shampoo Containing Candlenut (Aleurites moluccanus) Oil for Hair Health]. J Teknol Kim Unimal. 2022;11(1):32-42. doi: 10.29103/jtku.v11i1.7247
40. Mariska RP, Agung MH, Ibriyanti S. Formulasi dan Evaluasi Sifat Fisik Sediaan Shampo dari Ekstrak Daun Sungkai (Peronema canescens Jack) [Formulation and Evaluation of the Physical Properties of a Shampoo Containing Sungkai (Peronema canescens Jack) Leaf Extract]. J Akad Baiturrahim Jambi. 2025;14(September):403-414. doi: 10.36565/jab.v14i2.959
41. Kumar J, Azmal M, Faruk O, Shah ANM, Been N, Meem M, Ghosh A. Unlocking the secrets of the hair microbiome : From scalp health to therapeutic advances. The Microbe. 2025;7(April):100353. doi: 10.1016/j.microb.2025.100353
42. Deng L, Ling X, Li L, He Y, Guo M. Advances in Scalp Microbiome Research : Molecular Insights into the Metabolism-Inflammation-Barrier Axis and Dandruff Pathogenesis. Molecules. 2026;31(2093):1-29. doi: 10.3390/molecules31122093
43. Kartika DA, Legowo AM, Nurwantoro N. Pengaruh Penambahan Kurma terhadap Kadar Air, Derajat Keasaman (pH), Total Padatan Terlarut, dan Viskositas pada Yogurt Sari Kacang Hijau [Effect of Date Fruit Addition on Moisture Content, Acidity (pH), Total Soluble Solids, and Viscosity of Mung Bean Milk Yogurt]. J Teknol Pangan. 2025;9(2):64-69. doi: 10.14710/jtp.2025.51501
44. C. J. Thompson, N. Ainger, P. Starck, O. O. Mykhaylyk, A. J. Ryan, Shampoo Science: A Review of the Physiochemical Processes behind the Function of a Shampoo. Macromol. Chem. Phys. 2023, 224, 2200420. doi: 10.1002/macp.202200420
45. Thomas NA, Tungadi R, Ramadani D, Papeo P, Manoppo YS. Pengaruh Variasi Konsentrasi Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa) terhadap Stabilitas Fisik Sediaan Krim [The Effect of Varying Concentrations of Mahkota Dewa (Phaleria macrocarpa) Fruit Extract on the Physical Stability of a Cream Formulation]. Indones J Pharm Educ. 2022;2(2):143-152. doi: 10.37311/ijpe.v2i2.13532
46. Zhang Z, McClements DJ. Chapter 2 - Overview of Nanoemulsion Properties: Stability, Rheology, and Appearance. In: Jafari SM, McClements DJBTN, eds. Nanoemulsions. Academic Press; 2018:21-49. doi: 10.1016/B978-0-12-811838-2.00002-3
47. Akbari S, Nour AH. Emulsion types, stability mechanisms and rheology : A review. Int J Innov Res Sci Stud. 2018;1(September):14-21. doi: 10.53894/ijirss.v1i1.4
48. Lestari L, Nita P, Tegar M. Formulasi dan Karakterisasi Nanopartikel Kitosan Ekstrak Daun Dadap Serep (Erythrina subumbrans) sebagai Kandidat Herbal Antimastitis [The Formulation and Characterization of Chitosan Nanoparticles from Dadap Serep (Erythrina Subumbrans) Leaf Extract as Antimastitic Herbal Candidate]. J Kesehat Madani Med. 2024;15(01):94-105. doi: 10.36569/jmm.v15i01.357
49. Tungadi R, Thomas NA, Gobel WG Van. Formulasi, Karakterisasi, dan Evaluasi Drops Liquid Self Nano- Emulsifying Drug Delivery System (SNEDDS) Astaxanthin [Formulation, Characterization, and Evaluation of Liquid Self-Nano-Emulsifying Drug Delivery System (SNEDDS) Drops Containing Astaxanthin]. Indones J Pharm Educ. 2021;1(3):168-178. doi: 10.37311/ijpe.v1i3.11400
50. Wilson RJ, Li Y, Yang G, Zhao C xia. Nanoemulsions for drug delivery. Particuology. 2022;64:85-97. doi: 10.1016/j.partic.2021.05.009
51. Zhang Z, McClements DJ. Overview of nanoemulsion properties: stability, rheology, and appearance. In: Jafari SM, McClements DJ, eds. Nanoemulsions. Academic Press; 2018:21-49. doi: 10.1016/B978-0-12-811838-2.00002-3
52. Sandhya M, Ramasamy D, Sudhakar K, Kadirgama K, Harun WSW. Ultrasonication an intensifying tool for preparation of stable nanofluids and study the time influence on distinct properties of graphene nanofluids – A systematic overview. Ultrason Sonochem. 2021;73:105479. doi: 10.1016/j.ultsonch.2021.105479
53. Yuhara NA, Immanuel UK. Formulasi dan Uji Aktivitas Anti Ketombe P. Ovale Shampo Ekstrak Etanol Daun Murbei (Morus alba L.) [Formulation and Evaluation of the Anti-Dandruff Activity of Shampoo Containing Ethanolic Extract of Mulberry Leaves (Morus alba L.) Against Pityrosporum ovale]. J Mhs Ilmu Farm dan Kesehat. 2024;2(1):116-125. doi: 10.59841/jumkes.v2i1.877
54. Draelos ZD. Front Matter. In: Cosmetic Dermatology. ; 2022:i-xiv. doi: 10.1002/9781119676881.fmatter
55. Kurniaji A, Idris M, Muliani. Uji Daya Hambat Ekstrak Daun Mangrove (Sonneratia alba) pada Bakteri Vibrio harveyi secara In Vitro [In Vitro Evaluation of the Antibacterial Activity of Mangrove (Sonneratia alba) Leaf Extract Against Vibrio harveyi]. J Sains Teknol Akuakultur. 2021;3:84-92. https://core.ac.uk/outputs/267827180/
56. Rahayu KM, Chairani A. Efektivitas Anti Bakteri Ekstrak Daun Jengkol (Pithecellobiumjiringa) Terhadap Pertumbuhan Salmonellatyphimurium : Studi In Vitro Menggunakan Metode Difusi Cakram [In Vitro Evaluation of the Antibacterial Activity of Pithecellobium jiringa Leaf Extract Against Salmonella Typhimurium Using the Disk Diffusion Method]. Biocaster J Kaji Biol. 2025;5(3):287-297. doi: 10.36312/biocaster.v5i3.477
57. Soni S, Noor U, Sharma A, Tripathi SM, Sundaram S, Gupta E. Assessment of antimicrobial potential of polyphenol-rich Ananas comosus peel powder at different drying conditions. Indian Journal of Natural Products and Resources, 2025;16(3):417-427. doi: 10.56042/ijnpr.v16i3.15228
58. Nugraha IS, Udayani N, Aditama IGAS. Pengaruh Pemberian Elektrolit Buah Nanas terhadap Denyut Nadi Siswa SMK saat Melakukan Olahraga [Effect of Pineapple Fruit Electrolyte Supplementation on Heart Rate in Vocational High School Students During Physical Exercise]. J Edukasi Citra Olahraga. 2025;5:1-9. doi: 10.38048/jor.v4i3.4796
59. Mursidah A, Salam S, Agustina R. Profil Fitokimia dan Uji Aktivitas Antibakteri Ekstrak Kulit Buah Nanas (Ananas comosus (L.) Merr) [Phytochemical Profiling and Antibacterial Activity Evaluation of Pineapple (Ananas comosus (L.) Merr.) Peel Extract]. Proceeding Mulawarman Pharm Conf. 2023;18:1-6. doi: 10.25026/mpc.v18i1.695
60. Rahmawati I, Maulida R, Aisyah S. Potensi Antibakteri Sediaan Sabun Cair Ekstrak Kulit Nanas (Ananas comosus L. Merr.) Terhadap Bakteri Staphylococcus aureus ATCC 25923 [Antibacterial Potential of Liquid Soap Preparation Pineapple Peel Extract (Ananas comosus L. Merr.) Against Staphylococcus aureus ATCC 25923]. J Farm Sains Indones. 2021;4:1-11. doi: 10.52216/jfsi.vol4no2p1-11
61. Castro, A. C. L.; Lubido, A. C. C.; Balderas, A. K. A.; Gonzales, R. K. P.; Saldo, I. J. P. Phytochemical Analysis and Antibacterial Assessment of Pineapple (Ananas Comosus) Peel Extract. S. Asian Res. J. Nat. Prod. 2025;8:1: 153-161. doi: 10.9734/sarjnp/2025/v8i1188
62. De Rossi, L., Rocchetti, G., Lucini, L., & Rebecchi, A. Antimicrobial Potential of Polyphenols: Mechanisms of Action and Microbial Responses—A Narrative Review. Antioxidants, 2025:14(2), 200. doi: 10.3390/antiox14020200.
63. Nurnaningsih H, Laela DS. Efektivitas daya antibakteri berbagai konsentrasi enzim bromelain dari ekstrak buah nanas Ananas comosus ( L .) Merr . terhadap Streptococcus mutans secara in-vitro [The antibacterial activity effectiveness of various concentrations of bromelain enzymes from pineapple (Ananas comosus (L.) Merr.) extract on Streptococcus mutans in-vitro]. Padjadjaran J Dent Res Students. Published online 2022. doi: 10.24198/pjdrs.v6i1.38211
64. Pradita D, Pertiwi I, Limbong YS. Tinjauan Literatur: Mekanisme Antibakteri Ekstrak Kulit Jeruk Purut (Citrus hystrix DC.) [Antibacterial Mechanisms of Kaffir Lime (Citrus hystrix DC.) Peel Extract: A Literature Review]. J Indah Sains dan Klin. 2025;06(01):26-39. doi: https://doi.org/10.52622/jisk.v6i1.04
65. Rosida A, Hujjatusnaini N, Adah A. Flavonoid Profile of Peperomia pellucida Extract and Its Potential for Ophthalmic Therapy. J Syifa Sci Clin Res. 2025;7(3):412-422. doi: 10.37311/jsscr.v7i3.34643
66. Celine RG, Muhammad HM, Sujit B, Vincent HT, Kendra PR, Wei L, Xinli L; Nanoemulsion delivery systems for enhanced efficacy of antimicrobials and essential oils. Biomater. Sci. 2022;10(3):633–653. doi: 10.1039/d1bm01537k
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