Chitosan-Based Electrospun Nanofibers for Wound Healing: A Systematic and Bibliometric Synthesis of Material Design and Biological Performance
DOI:
https://doi.org/10.36733/medicamento.v12i2.14768Keywords:
bibliometric analysis, chitosan, electrospinning, nanofiber, systematic review, wound healingAbstract
Background: Chronic wound pathogenesis is associated with infection, inflammation, oxidative stress, and poor tissue regeneration, creating a major clinical challenge. Electrospun nanofibers, especially using chitosan, may be promising therapy with various advantages.
Objective: This research investigated design strategies and methodological factors affecting the therapeutic efficacy and translational potential of chitosan-based electrospun nanofibers for wound healing.
Methods: Advances in the research field, formulation characteristics, production specifications, biological efficacy, and comparability between studies were evaluated through a systematic analysis of 23 studies and a bibliometric analysis of 186 articles published between 2015 and 2025.
Results: The annual growth rate of publications is 29.24%, indicating growing interest in the methods of multifunctional wound-dressing methods. This allows evaluation of different studies to understand the characteristics of chitosan for electrospinning. Most studies employed single-layer nanofiber scaffolds incorporating medium- to high- molecular weight chitosan, with varying copolymer, bioactive, and crosslinker compositions, as well as electrospinning parameters, particularly applied voltage and flow rate. However, differences in production processes, biological assessment methods, and wound models limited the comparability of the studies. Healing outcomes were more dependent on mechanism-based approaches that addressed the underlying causes of injuries.
Conclusion: The results demonstrate that differences in manufacturing conditions, material composition, and biological assessment can strongly affect the interpretation across studies.
References
1. Sen CK. Human Wound and Its Burden: Updated 2025 Compendium of Estimates. Advances in Wound Care. 2025;14(9):429-438. doi:10.1177/21621918251359554
2. Díaz-Herrera MÁ, González-Durán M, Rodríguez-Martínez FJ, Tujillo-Flores G, Tuset-Mateu N, Verdu-Soriano J, Gea-Caballero V, Sanllorente-Melenchon A, Almenda-Ortega J, Cunillera-Puertolas O, Acedo-Anta M, Matinez-Riera JR. The financial burden of chronic wounds in primary care: A real-world data analysis on cost and prevalence. Int J Nurs Stud Adv. 2025;8:100313. doi:10.1016/j.ijnsa.2025.100313
3. Elajaili H, Lyttle BD, Lewis CV, Bardill JR, Dee N, Seal S, Nozik ES, Liechty KW, Zgheib C. Increased ROS and Persistent Pro-Inflammatory Responses in a Diabetic Wound Healing Model (db/db): Implications for Delayed Wound Healing. Int J Mol Sci. 2025;26(10):4884. doi:10.3390/ijms26104884
4. Song J, Zhao T, Wang C, Sun X, Sun J, Zhang Z. Cell migration in diabetic wound healing: Molecular mechanisms and therapeutic strategies (Review). Int J Mol Med. 2025;56(2):126. doi:10.3892/ijmm.2025.5567
5. Phan S, Feng CH, Huang R, Lee ZX, Moua Y, Phung OJ, Lenhard JR. Relative Abundance and Detection of Pseudomonas aeruginosa from Chronic Wound Infections Globally. Microorganisms. 2023;11(5):1210. doi:10.3390/microorganisms11051210
6. Lu X, Zhou L, Song W. Recent Progress of Electrospun Nanofiber Dressing in the Promotion of Wound Healing. Polymers. 2024;16(18). doi:10.3390/polym16182596
7. Ali SH, Mahammed MA, Yasin SA. Characterization of Electrospinning Chitosan Nanofibers Used for Wound Dressing. Polymers. 2024;16(14). doi:10.3390/polym16141984
8. Nasaj M, Chehelgerdi M, Asghari B, Ahmadieh-Yazdi A, Asgari M, Kabiri-Samani S, Sharifi E, Arabestani M. Factors influencing the antimicrobial mechanism of chitosan action and its derivatives: A review. International Journal of Biological Macromolecules. 2024;277:134321. doi:10.1016/j.ijbiomac.2024.134321
9. Tripathi D, Rajinikanth PS, Pandey P. Electrospun chitosan nanofibers for regenerative wound healing: from molecular design to functional scaffolds. J Mater Chem B. 2025;13(35):10743-10779. doi:10.1039/D5TB01405K
10. Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffman TC, Mulrow CD, Shamseer L, Tetzlaff JM, Akl EA, Brennan SE, Chou R, Glanville J, Grimshaw JM, Hrobjartsson A, Lalu MM, Li T, Loder EW, Mayo-Wilson E, McDonald S, McGuinness LA, Stewart LA, Thomas J, Tricco AC, Welch VA, Whiting P, Moher D. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ. 2021;372:n71. doi:10.1136/bmj.n71
11. Aria M, Cuccurullo C. Bibliometrix: An R-tool for comprehensive science mapping analysis. Journal of Informetrics. 2017;11(4):959-975. doi:10.1016/j.joi.2017.08.007
12. van Eck NJ, Waltman L. Software survey: VOSviewer, a computer program for bibliometric mapping. Scientometrics. 2010;84(2):523-538. doi:10.1007/s11192-009-0146-3
13. Qasim SB, Zafar MS, Najeeb S, Khurshid Z, Shah AH, Husain S, Rehman IU. Electrospinning of Chitosan-Based Solutions for Tissue Engineering and Regenerative Medicine. Int J Mol Sci. 2018;19(2):407. doi:10.3390/ijms19020407
14. Tamilarasi GP, Sabarees G, Manikandan K, Gouthaman S, Alagarsamy V, Solomon VR. Advances in electrospun chitosan nanofiber biomaterials for biomedical applications. Mater Adv. 2023;4(15):3114-3139. doi:10.1039/D3MA00010A
15. Venable GT, Shepherd BA, Loftis CM, McClatchy SG, Roberts ML, Fillinger ME, Tansey JB, Klimo P Jr. Bradford’s law: identification of the core journals for neurosurgery and its subspecialties. Published online February 1, 2016. doi:10.3171/2015.3.JNS15149
16. Sánchez-Pita F, Benito-Cabello M, Puebla-Martínez B. Editorial Policy and the Dissemination of Scientific Knowledge on Open Access—Case Study: Science Communication Journals in Latin America. Publications. 2025;13(3). doi:10.3390/publications13030039
17. Kokabisaghi F, Miller AC, Bashar FR, Salesi M, Zarchi AAK, Keramatfar A, Pourhoseingholi MA, Amini H, Vahedian-Azimi A. Impact of United States political sanctions on international collaborations and research in Iran. BMJ Glob Health. 2019;4(5). doi:10.1136/bmjgh-2019-001692
18. Scarazzati S, Wang L. The effect of collaborations on scientific research output: the case of nanoscience in Chinese regions. Scientometrics. 2019;121(2):839-868. doi:10.1007/s11192-019-03220-x
19. Abadehie FS, Dehkordi AH, Zafari, Bagheri M, Yousefiasl S, Pourmotobed S, Mahmoodnia L, Validi M, Ashrafizadeh M, Zare EN, Rabiee, Makvandi P, Sharifi E. Lawsone-encapsulated chitosan/polyethylene oxide nanofibrous mat as a potential antibacterial biobased wound dressing. Engineered Regeneration. 2021;2:219-226. doi:10.1016/j.engreg.2022.01.001
20. Zhang H, Zhang M, Wang X, Zhang M, Wang X, Li Y, Cui Z, Chen X, Han Y, Zhao W. Electrospun multifunctional nanofibrous mats loaded with bioactive anemoside B4 for accelerated wound healing in diabetic mice. Drug Deliv. 2022;29(1):174-185. doi:10.1080/10717544.2021.2021319
21. Fu J, Wang D, Tang Z, Xu Y, Xie J, Chen R, Wang P, Zhong Q, Ning Y, Lei M, Mai H, Li H, Liu H, Wang J, Cheng H. NIR-responsive electrospun nanofiber dressing promotes diabetic-infected wound healing with programmed combined temperature-coordinated photothermal therapy. Journal of Nanobiotechnology. 2024;22(1):384. doi:10.1186/s12951-024-02621-2
22. Xie J, Liu G, Chen R, Wang D, Mai H, Zhong Q, Ning Y, Fu J, Tang Z, Xu Y, Li H, Lei M, Cheng H, Huang Y, Zhang Y. NIR-activated electrospun nanodetonator dressing enhances infected diabetic wound healing with combined photothermal and nitric oxide-based gas therapy. Journal of Nanobiotechnology. 2024;22(1):232. doi:10.1186/s12951-024-02474-9
23. Saghafi Y, Baharifar H, Najmoddin N, Asefnejad A, Maleki H, Sajjadi-Jazi SM, Bonkdar A, Shams F, Khoshnevisan K. Bromelain- and Silver Nanoparticle-Loaded Polycaprolactone/Chitosan Nanofibrous Dressings for Skin Wound Healing. Gels. 2023;9(8):672. doi:10.3390/gels9080672
24. Liu C, Zhu Y, Lun X, Sheng H, Yan A. Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing. Bioengineered. 2022;13(2):4328-4339. doi:10.1080/21655979.2022.2031415
25. Bhardwaj D, Chawla V, Nandwani V, Thakur Y, Singh Y, Agrawal G. A multifunctional electrospun nanofiber/hydrogel-based pro-healing bilayer dressing as a next generation biomaterial for skin wound care. Journal of Materials Chemistry B. 2025;13(37):11821-11834. doi:10.1039/d5tb00800j
26. Ahmadabad MJ, Janmohammadi M, Nazemi Z, Bahraminasab M, Arab S, Nourbakhsh MS. Electrospun and freeze-dried PCL/chitosan-gelatin bilayer dressing for enhanced wound healing. Carbohydrate Polymer Technologies and Applications. 2025;12:101016. doi:10.1016/j.carpta.2025.101016
27. Aker SD, Tamburaci S, Tihminlioglu F. Development of Cissus quadrangularis-Loaded POSS-Reinforced Chitosan-Based Bilayer Sponges for Wound Healing Applications: Drug Release and In Vitro Bioactivity. ACS Omega. 2023;8(22):19674-19691. doi:10.1021/acsomega.3c00999
28. Ali IH, Ouf A, Elshishiny F, Taskin MB, Song J, Dong M, Chen M, Siam R, Mamdouh W. Antimicrobial and Wound-Healing Activities of Graphene-Reinforced Electrospun Chitosan/Gelatin Nanofibrous Nanocomposite Scaffolds. ACS Omega. 2022;7(2):1838-1850. doi:10.1021/acsomega.1c05095
29. Almasi L, Arkan E, Farzaei MH, Iranpanah A, Jalili C, Abbaszadeh F, Aghaz F, Fakhri S. Preparation of Tragopogon graminifolius-loaded electrospun nanofibers and evaluating its wound healing activity in a rat model of skin scar. Frontiers in Pharmacology. 2025;16:1533010. doi:10.3389/fphar.2025.1533010
30. Cam ME, Ertas B, Alenezi H, Hazar-Yavuz AN, Cesur S, Ozcan GS, Ekentok C, Guler E, Katsakouli C, Demirbas Z, Akakin D, Sayip M, Eroglu E, Kabasakal L, Gunduz O, Edirisinghe M. Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study. Materials Science and Engineering: C. 2020;119:111586. doi:10.1016/j.msec.2020.111586
31. Cheng Y, Far BF, Jahanbakhshi M, Bahrami S, Tamimi P, Sedaghat M, Ghazizadeha E. Exploring the potential of a polyvinyl alcohol/chitosan-based nanofibrous matrix for erythromycin delivery: fabrication, in vitro and in vivo evaluation. RSC Advances. 2023;13(27):18450-18460. doi:10.1039/d3ra02987e
32. Hashemi SS, Najari M, Parvin M, Mehdi M, Kalani K, Assadi M, Seyedian R, Zaeri S. Wound healing effects of dexpanthenol-loaded core/shell electrospun nanofibers: Implication of oxidative stress in wound healing. Iranian Journal of Basic Medical Sciences. 2024;27(1):97-106. doi:10.22038/ijbms.2023.71412.15526
33. Lv H, Zhao M, Li Y, Li K, Chen S, Zhao W, Wu S, Han Y. Electrospun Chitosan–Polyvinyl Alcohol Nanofiber Dressings Loaded with Bioactive Ursolic Acid Promoting Diabetic Wound Healing. Nanomaterials. 2022;12(17):2933. doi:10.3390/nano12172933
34. Maleki H, Doostan M, Khoshnevisan K, Baharifar H, Maleki SA, Fatahi MA. Zingiber officinale and thymus vulgaris extracts co-loaded polyvinyl alcohol and chitosan electrospun nanofibers for tackling infection and wound healing promotion. Heliyon. 2023;10(1):e23719. doi:10.1016/j.heliyon.2023.e23719
35. Monavari M, Sohrabi R, Motasadizadeh H, Monavari M, Fatahi Y, Ejarestaghi NM, Fuentes-Chandia M, Leal-Egana A, Akrami M, Homaeigohar S. Levofloxacin loaded poly (ethylene oxide)-chitosan/quercetin loaded poly (D,L-lactide-co-glycolide) core-shell electrospun nanofibers for burn wound healing. Frontiers in Bioengineering and Biotechnology. 2024;12:1352717. doi:10.3389/fbioe.2024.1352717
36. Saghebasl S, Amini H, Nobakht A, Haiaty S, Bagheri HS, Hasanpour P, Milani M, Saghati S, Naturi O, Farhadi M, Rahbarghazi R. Polyurethane-based nanofibrous mat containing porphyrin with photosensitivity and bactericidal properties can promote cutaneous tissue healing in rats. Journal of Nanobiotechnology. 2023;21(1):313. doi:10.1186/s12951-023-02082-z
37. Sethuram L, Chandrasekaran N. PDGF-loaded eugenol-impregnated biocompatible nanofibrous scaffolds for enhanced diabetic wound healing and vascularization. Materials Advances. 2025;6(24):9437-9464. doi:10.1039/d5ma00322a
38. Tavallaei O, Doostan M, Khoshnevisan K, Tahmasebi A, Maleki H. Wound healing promotion by poly(vinyl alcohol)/chitosan electrospun nanofibrous scaffold loaded with Achillea wilhelmsii extract. Nanomedicine Journal. 2025;12(1):110-121. doi:10.22038/nmj.2024.79495.1963
39. Tujjohra F, Islam M, Rashid TU, Rahman MM. Valorization of hazardous leather shaving dust for the fabrication of electrospun collagen/PVA/chitosan nanomats in wound care. Carbohydrate Polymer Technologies and Applications. 2025;10:100748. doi:10.1016/j.carpta.2025.100748
40. Wang Y, Zhao G. Novel fabrication and development of multifunctional Zn/Fu@Cs nanofibers material for wound care and operate room infection control. Materials Research Express. 2023;10(1):015002. doi:10.1088/2053-1591/ac9bd3
41. Wang L, Zhao LY, Tang WJ, Wang ZY, An MW, Liu Y, Wen ML, Wei Y. Near-infrared light-responsive photothermal-chemical synergistic antimicrobial GO-BPEI/La nanofiber membrane in the repair of skin wound inflammation. Medicine in Novel Technology and Devices. 2025;28:100397. doi:10.1016/j.medntd.2025.100397
42. Oviedo M, Montoya Y, Agudelo W, García-García A, Bustamante J. Effect of Molecular Weight and Nanoarchitecture of Chitosan and Polycaprolactone Electrospun Membranes on Physicochemical and Hemocompatible Properties for Possible Wound Dressing. Polymers (Basel). 2021;13(24):4320. doi:10.3390/polym13244320
43. Younes I, Sellimi S, Rinaudo M, Jellouli K, Nasri M. Influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities. International Journal of Food Microbiology. 2014;185:57-63. doi:10.1016/j.ijfoodmicro.2014.04.029
44. Ardean C, Davidescu CM, Nemeş NS, Negrea A, Ciopec M, Duteanu N, Negrea P, Duda Seiman D, Musta V. Factors Influencing the Antibacterial Activity of Chitosan and Chitosan Modified by Functionalization. International Journal of Molecular Sciences. 2021;22(14). doi:10.3390/ijms22147449
45. Foster LJR, Ho S, Hook J, Basuki M, Marçal H. Chitosan as a Biomaterial: Influence of Degree of Deacetylation on Its Physiochemical, Material and Biological Properties. PLOS ONE. 2015;10(8):e0135153. doi:10.1371/journal.pone.0135153
46. Wang S, Li J, Wang P, Zhang M, Liu S, Wang R, Li Y, Ren F, Fang B. Improvement in the Sustained-Release Performance of Electrospun Zein Nanofibers via Crosslinking Using Glutaraldehyde Vapors. Foods. 2024;13(10). doi:10.3390/foods13101583
47. Shi J, Lian H, Huang Y, Zhao D, Wang H, Wang C, Li J, Ke L. In vitro genotoxicity evaluation and metabolic study of residual glutaraldehyde in animal-derived biomaterials. Regen Biomater. 2020;7(6):619-625. doi:10.1093/rb/rbaa041
48. Witkowska K, Paczkowska-Walendowska M, Nagalska M, Miklaszewski A, Karpinski TM, Plech T, Espinnar FJO, Cielecka-Piontek J. Chitosan/PEO Nanofibers as a Delivery Platform for Sustained Release of Centella asiatica Extract. International Journal of Molecular Sciences. 2025;26(24):12134. doi:10.3390/ijms262412134
49. Bonakdar MA, Rodrigue D. Electrospinning: Processes, Structures, and Materials. Macromol. 2024;4(1):58-103. doi:10.3390/macromol4010004
50. Mailley D, Hébraud A, Schlatter G. A Review on the Impact of Humidity during Electrospinning: From the Nanofiber Structure Engineering to the Applications. Macromolecular Materials and Engineering. 2021;306(7):2100115. doi:10.1002/mame.202100115
51. Mouro C, Fangueiro R, Gouveia IC. Preparation and Characterization of Electrospun Double-Layered Nanocomposites Membranes as a Carrier for Centella asiatica (L.). Polymers. 2020;12(11):2653. doi:10.3390/polym12112653
52. Perpelek M, Tamburaci S, Karakasli A, Tihminlioglu F. Fabrication of Bioactive Helix aspersa Extract-Loaded Chitosan-Based Bilayer Wound Dressings for Skin Tissue Regeneration. ACS Omega. 2024;9(49):48070-48088. doi:10.1021/acsomega.4c04345
53. Sahariah P, Kontogianni GI, Scoulica E, Sigurjonsson OE, Chatzinikolaidou M. Structure-activity relationship for antibacterial chitosan carrying cationic and hydrophobic moieties. Carbohydrate Polymers. 2023;312:120796. doi:10.1016/j.carbpol.2023.120796
54. Molina MIE, Malollari KG, Komvopoulos K. Design Challenges in Polymeric Scaffolds for Tissue Engineering. Front Bioeng Biotechnol. 2021;9. doi:10.3389/fbioe.2021.617141
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