Proliferation of Nano Chitosan and Platelet Rich Plasma from Pre-osteoblast Cell with Ki67 as a Surrogate Marker in Vitro

Dewa Made Wedagama

doi:10.36733/medicamento.v11i2.11435

Penulis

Dewa Made Wedagama Universitas Mahasaraswati Denpasar

DOI:

https://doi.org/10.36733/medicamento.v11i2.11435

Kata Kunci:

hydroksiapatit, nano-chitosan, proliferasi, PRP, sel preosteoblas

Abstrak

Penelitian ini bertujuan untuk menyelidiki efek campuran nano-chitosan dengan plasma kaya platelet (PRP) terhadap laju proliferasi sel pre-osteoblast dalam sistem kultur in vitro dengan waktu inkubasi yang digunakan. Medium kultur sel pre-osteoblast MC3T3-E1 menggunakan alpha-MEM, 2 mm L-glutamin, 1 mm natrium piruvat, 10% FBS, dan 10% penisilin-streptomisin dalam botol flask 25 cm² dan diinkubasi dalam inkubator dengan 5% CO₂ pada suhu 37°C hingga sel mencapai kepadatan 70-80% dan ditanam dalam plate 24 sumur untuk pemberian perlakuan. Perlakuan dibagi menjadi dua kelompok, nano-chitosan+PRP dan hydroxyapatite+PRP. Proliferasi sel pre-osteoblast diamati dengan pewarnaan imunositokimia, dan jumlah sel yang berkembang biak dihitung dan dianalisis menggunakan mikroskop laser pemindaian konfokal (CLSM). Normalitas data sampel dianalisis dengan uji Shapiro-Wilk. Uji perbandingan menggunakan uji t sampel independen dan ANOVA satu arah (uji F). Semua data dianalisis menggunakan perangkat lunak SPSS. Hasil eksperimen menunjukkan bahwa nano-chitosan+PRP dapat mempercepat proliferasi dibandingkan dengan hidroksiapatit+PRP pada konsentrasi 0% dan 10%. Uji t sampel independen menunjukkan adanya perbedaan yang signifikan (p=0.010<α) antara rata-rata laju proliferasi (0%) pada kelompok perlakuan nano-chitosan+PRP (1076.3±176.4au) dan kelompok perlakuan hidroksiapatit+PRP (659,5±272,7au) pada waktu inkubasi lima hari, serta rata-rata proliferasi (10%) antara kelompok perlakuan nano-chitosan+PRP (710,3±109,7au) dan hidroksiapatit+PRP (581,8±76,4au) pada waktu inkubasi tujuh hari. Berdasarkan rata-rata proliferasi (0%) dan (10%), kelompok perlakuan nano-chitosan+PRP dengan waktu inkubasi lima dan tujuh hari memiliki rata-rata yang lebih tinggi daripada 0% dan 10% pada kelompok perlakuan nano-chitosan dan PRP, dan dapat mempercepat penyembuhan tulang dengan waktu inkubasi lima dan tujuh hari dibandingkan dengan kelompok perlakuan hidroksiapatit+PRP.

Biografi Penulis

Dewa Made Wedagama, Universitas Mahasaraswati Denpasar

Fakultas Kedokteran Gigi

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