The Effect of Probiotic Yogurt and Fermented Skim Milk Beverages on The Reduction of Streptococcus Mutans In Children's Dental Caries

Authors

  • Eko Sri Yuni Astuti Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali, Indonesia
  • Putu Ariska Indraswari Undergraduate Program, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali, Indonesia
  • Putu Widani Astuti Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali, Indonesia
  • Yudha Rahina Department of Public Dental Health and Prevention, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Bali, Indonesia

DOI:

https://doi.org/10.46862/interdental.v20i2.9117

Keywords:

Artificial saliva, probiotics, skim milk-based fermented beverage, Streptococcus mutans, yogurt

Abstract

Introduction: Dental caries is the most common dental and oral problem in children, with a prevalence of 90.2% in children under 6 years old . Streptococcus mutans is considered one of the most virulent caries-causing microorganisms due to its ability to colonize and initiate plaque formation on tooth surfaces. Probiotic yogurt and skim milk-based fermented drinks, which are popular with children, can potentially inhibit the growth of pathogenic oral bacteria. This study aimed to determine the effect of these probiotic drinks on reducing Streptococcus mutans counts in pediatric dental caries.

Materials and Methods: This in vitro laboratory study used a post-test only control group design. Streptococcus mutans colonies obtained from human dental plaque were immersed in artificial saliva with yogurt for 4 minutes, skim milk-based fermented drinks for 2 minutes, or distilled water (negative control) for 2 or 4 minutes. Bacterial growth was observed and analyzed statistically.

Results: Both probiotic yogurt drinks and skim milk-based fermented drinks reduced the number of Streptococcus mutans bacteria.

Conclusion: Skim milk-based fermented beverages showed a greater reduction in Streptococcus mutans compared to yogurt. Poor oral hygiene practices, cariogenic diets, and low socioeconomic status are reported risk factors for dental caries in children. Regular tooth brushing and limiting sugar consumption can help prevent caries development.

Downloads

Download data is not yet available.

References

Tim Riskesdas 2018. Laporan Nasional RISKESDAS 2018. Kementrian Kesehatan RI Badan Penelitian dan Pengembangan Kesehatan. Jakarta: Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan; 2019. h. 204-205.

Siddiqui M, Singh C, Masih U, Chaudhry K, Hegde DY, Gojanur S. Evaluation of Streptococcus mutans levels in saliva before and after consumption of probiotic milk: A Clinical Study. Journal of International Oral Health 2016; 8(2):195-198.

Babaji P, Keswani K, Lau H, Lau M, Sharma N, Punga R. Role of probiotics in oral health: A review of the literature. Journal of Education and Ethics in Dentistry 2012; 2(2):52-55. Doi: https://doi.org/10.4103/0974-7761.121256

Coqueiro AY, Bonvini A, Raizel R, Tirapegui J, Rogero MM. Probiotic Supplementation In Dental Caries: Is It Possible To Replace Conventional Treatment?. Nutrire 2018; 43(1):1-9. Doi: https://doi.org/10.1186/s41110-018-0064-3

Jill CMR. Kids yogurt: how to choose the best one. Tersedia di: https://thenourishedchild.com/best-kids-yogurt/. Diakses pada: 17 Agustus 2020.

Glavina D, Goršeta K, Škrinjarić I, Negovetić VD, Mehulić K, Kožul K. Effect of LGG yoghurt on Streptococcus mutans and Lactobacillus Spp. salivary counts in children. Collegium antropologicum 2012; 36(1):129-132.

Sutula J, Coulthwaite LA, Thomas, LV, Verran J. The effect of a commercial probiotic drink containing lactobacillus casei strain shirota on oral health in healthy dentate people. Microbial Ecology In Health And Disease 2013; 24(1):1-12. Doi: 10.3402/mehd.v24i0.21003

Lin X, Chen X, Tu Y, Wang S, Chen H. Effect of probiotic lactobacilli on the growth of Streptococcus mutans and multispecies biofilms isolated from children with active caries. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research 2017; 23:4175–4181. Doi: 10.12659/msm.902237

Payadnya IPAA, Jayantika IGANT. Panduan penelitian eksperimen beserta analisis statistik dengan SPSS. Yogyakarta: Deepublish; 2018.

Dan T, Wang D, Wu S, Jin R, Ren W, Sun T. Profiles of volatile flavor compounds in milk fermented with different proportional combinations of Lactobacillus delbrueckii subsp. Bulgaricus and Streptococcus thermophilus, Molecules 2017; 22(10):1-14. Doi: https://doi.org/10.3390/molecules22101633

Ilyas M, Phielip C. Konsumsi yogurt menurunkan jumlah koloni bakteri kariogenik dalam saliva pada usia remaja. Makassar Dental Journal 2012; 1(1):1-8. Doi: https://doi.org/10.35856/mdj.v1i1.42

Nikolov P. Probiotics and mucosal immune respone. in e.c. rigobelo (ed). Probiotics. IntechOpen 2012; 481-98 http://doi.org/http://dx.doi.org/10.5772/50042

Yadav M, Poornima P, Roshan NM, Prachi N, Veena M, Neena IE. Evaluation of probiotic milk on salivary Mutans Streptococci count: An in vivo microbiological study. Journal of Clinical Pediatric Dentistry 2014; 39(1):23-26. Doi: 10.17796/jcpd.39.1.u433n8w245511781

Caglar E, Kuscu OO, Selvi Kuvvetli S, Kavaloglu Cildir S, Sandalli N, Twetman S. Short-term effect of ice-cream containing Bifidobacterium-lactis Bb-12 on the number of salivary mutans streptococci and lactobacilli. Acta Odontol Scand 2008; 66(3):154-8. Doi: 10.1080/00016350802089467

Tong Z, Zhou L, Li J, Kuang R, Lin Y, Ni L. An in vitro investigation of Lactococcuslactis antagonizing cariogenic bacterium Streptococcus mutans. Arch Oral Biol 2012; 57(4): 376-82. Doi: 10.1016/j.archoralbio.2011.10.003

Dalie DKD, Deschamps, Richarrd FF. Lactic acid bacteria: potential for control of mould growth and mycotoxins: a review. Food Control 2010; 21:370-380. Doi: 10.1016/j.foodcont.2009.07.011

Nur F, Potensi bakteri asam laktat yang diisolasi dari dangke sebagai kandidat probiotik dalam menghambat bakteri patogen. Tesis. Pascasarjana Universitas Hasanudin; 2017.

Kumar A. Probiotics: Nature's Medicine. International Journal of Nutrition, Pharmacology, Neurological Diseases 2013; 3(3):219-228. Doi: 10.4103/2231-0738.114838

Bergman TL, Lavine AS, Incropera FP, DeWitt DP. Fundamentals of Heat and Mass Transfer. Hoboken, NJ: John Wiley & Sons; 2017

Cagetti MG, Mastroberardino S, Milia E, Cocco F, Lingström P, Campus G. The use of probiotic strains in caries prevention: A Systematic Review. Nutrients 2013; 5:2530-2550. https://doi.org/10.3390/nu5072530

Downloads

Published

2024-08-01

How to Cite

1.
Astuti ESY, Indraswari PA, Astuti PW, Rahina Y. The Effect of Probiotic Yogurt and Fermented Skim Milk Beverages on The Reduction of Streptococcus Mutans In Children’s Dental Caries. interdental [Internet]. 2024 Aug. 1 [cited 2024 Nov. 14];20(2):211-7. Available from: https://e-journal.unmas.ac.id/index.php/interdental/article/view/9117

Most read articles by the same author(s)