Antibacterial Effects of Tobacco Leaf Extract (Nicotiana tabacum) on Fusobacterium nucleatum (in vitro Study)
DOI:
https://doi.org/10.46862/interdental.v21i1.11388Keywords:
Fusobacterium nucleatum, Nicotiana tabacum leaf extract, antibacterial effects, MBCAbstract
Introduction: Deep caries can involve the pulp tissue, necessitating pulp capping treatment. Fusobacterium nucleatum is frequently found in deep caries. One of the essential requirements for pulp capping materials is antibacterial properties. Mineral Trioxide Aggregate (MTA) is a commonly used pulp capping material due to its favorable characteristics, such as antibacterial properties, biocompatibility, and more. However, MTA has several drawbacks, including a long setting time, difficult manipulation, risk of staining and discoloration, and high cost. Nicotiana tabacum leaf extract has the potential to serve as an herbal alternative for pulp capping materials, as it is known to possess antibacterial properties derived from its secondary metabolites. This study aimed to determine the antibacterial effects of Nicotiana tabacum leaf extract on Fusobacterium nucleatum. Materials and Methods: An in vitro experimental laboratory study with a post-test only control group design was conducted. The antibacterial test used the 96-well microdilution and plate count methods to determine Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). There were nine test groups, consisting of Nicotiana tabacum leaf extract at 3.125%, 6.25%, 12.5%, 25%, and 50% of 1000 µg/µL (31.25 µg/µL, 62.5 µg/µL, 125 µg/µL, 250 µg/µL, and 500 µg/µL), sterile aquadest (negative control), MTA (positive control), and internal controls (Nicotiana tabacum leaf extract and sterile aquadest).
Results: The number of bacterial colonies significantly decreased as the extract concentration increased. Nicotiana tabacum leaf extract showed MIC values at 12.5% (125 µg/µL) and MBC values at 25% (250 µg/µL). The antibacterial activity of Nicotiana tabacum leaf extract is attributed to its secondary metabolites, including flavonoids, saponins, steroids, and triterpenoids, which disrupt bacterial structures and functions.
Conclusion: Nicotiana tabacum leaf extract has antibacterial effects (bacteriostatic and bactericidal) on Fusobacterium nucleatum.
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References
Putri VS, Maimaznah M. Efektifitas gosok gigi massal dan pendidikan kesehatan gigi mulut pada anak usia 7-11 tahun di SDN 174 kel. Murni Kota Jambi. JAK 2021;3(1):63-71. doi: http://dx.doi.org/10.36565/jak.v3i1.152
Hidayah A, Kusuma Astuti I, Larasati R. Pengetahuan orang tua tentang karies gigi pada anak usia dini di TK Dewi Masyithoh Umbulsari Jember. IJOHM 2022;2(4):461-8.
Arba Kartika L, Hidayati S, Fitria Ulfah S. Gambaran pengetahuan tentang karies gigi pada siswa kelas 6 SDN Kertajaya I Surabaya. IJOHM 2021;1(1):32-40.
Utami S, Pinastika R, Astuti NR, Adiningrat A. The overview of eating patterns and dental caries status of the community of Pendul, Argorejo, Bantul Yogyakarta. IDJ 2023;12(1):1–6. doi: http://dx.doi.org/10.18196/di.v12i1.14749
Rathee M, Sapra A. Dental caries. In StatPearls [Internet]. 2023 Jun 21. Available from: https://www.ncbi.nlm.nih.gov/books/NBK551699/
Harahap AA, Melvin. Profil perawatan pulpa pada pasien anak di RSGMP FKG USU klinik IKGA tahun 2010-2015 [Internet]. Universitas Sumatera Utara; 2017 [cited 2024 Apr 27]. Available from: http://repositori.usu.ac.id/handle/123456789/19232
Octaviani NP, Mooduto L, Sudirman A. Antibacterial potency of mangosteen pericarp extracts (Garcinia mangostana L.) against Fusobacterium nucleatum. CDJ 2020;10(2):44-7. doi: https://doi.org/10.20473/cdj.v10i2.2020.44-47
Piyasoonthorn P, Mitrakul K. Association between Bifidobacterium, Fusobacterium nucleatum and type of root canal infections in primary teeth and clinical symptom: a quantitative real-time PCR analysis. SWU Dent J 2023;16(2):99-113.
Muchova M. Characterisation of Fusobacterium nucleatum biofilms and cyclic di-nucleotide production. Dissertation. University of Birmingham; 2020.
Pignatelli P, Nuccio F, Piattelli A, Curia MC. The role of Fusobacterium nucleatum in oral and colorectal carcinogenesis. Microorganisms. MDPI 2023;11(9):2358. doi: https://doi.org/10.3390/microorganisms11092358
Islam R, Islam MRR, Tanaka T, Alam MK, Ahmed HMA, Sano H. Direct pulp capping procedures – evidence and practice. Jpn Dent Sci Rev. Elsevier Ltd 2023;59:48–61. doi: https://doi.org/10.1016/j.jdsr.2023.02.002
Barrett B, O’Sullivan M. Management of the deep carious lesion: a literature review. J Ir Dent Assoc 2020;67(1):36–42.
Suliasih BA, Mun’im A. Chemistry and materials review: potensi dan masalah dalam pengembangan kemandirian bahan baku obat tradisional di Indonesia. Chem Mater 2022;1(1):28-33.
Lestari DA. Gambaran pengetahuan, kepercayaan masyarakat dan efek samping penggunaan obat herbal di Kabupaten Lampung Timur. Thesis. Jakarta: UIN Syarif Hidayatullah Jakarta; 2020 [cited 2024 Apr 28].
Pratiwi IA, Hidayah SAN, Violinita LNN. Efektifitas dana bagi hasil cukai hasil tembakau (DBH CHT) dalam mengatasi dampak pandemi Covid-19. JIA 2022;6(1):31–40. doi: https://doi.org/10.31092/jia.v6i1.1279
Pramono A, Amru BA, Rahmani HA, Fernanda SA, Nugraha Y, Ramadhan MYA, et al. Nicotiana tabacum var. Virginia bio oil-based pyrolisis extraction have prominence antimicrobial potential compared to ethanol heat reflux extraction (EHRE). Hayati J Biosci 2022;29(4):515–25. doi: http://dx.doi.org/10.4308/hjb.29.4.515-525
Hernández-Alvarado J, Zaragoza-Bastida A, López-Rodríguez G, Peláez- Acero A, Olmedo-Juárez A, Rivero-Perez N. Actividad antibacteriana y sobre nematodos gastrointestinales de metabolitos secundarios vegetales: enfoque en Medicina Veterinaria. Abanico Vet 2018;8(1):14–27.
Khasanah AU, Nastiti SJ. Identifikasi senyawa aktif ekstrak daun tembakau (Nicotiana tabacum L.) sebagai antibakteri terhadap S. aureus (ATCC 25923). Al-Hayat J Biol Appl Biol 2021;4(1):19–32. doi: https://doi.org/10.21580/ah.v4i1.6320
Putri RH, Barid I, Kusumawardani B. Daya hambat ekstrak daun tembakau terhadap pertumbuhan mikroba rongga mulut. Stomatognatik Jurnal Kedokteran Gigi 2014;11(2):27–31.
Lim M, Yoo S. The antibacterial activity of mineral trioxide aggregate containing calcium fluoride. J Dent Sci 2022;17(2):836–41. doi: https://doi.org/10.1016/j.jds.2021.09.005
Wendersteyt NV, Wewengkang DS, Abdullah SS. Uji aktivitas antimikroba dari ekstrak dan fraksi ascidian Herdmania momus dari perairan Pulau Bangka Likupang terhadap pertumbuhan mikroba Staphylococcus aureus, Salmonella typhimurium dan Candida albicans. Pharmacon 2021;10(1). doi: https://doi.org/10.35799/pha.10.2021.32758
Muiz HA, Wulandari S, Primadiamanti A. Uji aktivitas antibakteri ekstrak daun patikan kebo (Euphorbia hirta L) terhadap Staphylococcus aureus dengan metode difusi cakram. JAF 2022;6(2):84–9. doi: https://doi.org/10.33024/jaf.v6i2.5942
Gerung WHP, Fatimawali, Antasionasti I. Antibacterial activity test of belimbing botol leaf extract (Averrhoa bilimbi L.) against the growth of Propionibacterium acne. Pharmacon 2021;10(4):1087-1093. doi: https://doi.org/10.35799/pha.10.2021.37403
Handoyo DLY. Pengaruh lama waktu maserasi (perendaman) terhadap kekentalan ekstrak daun sirih (Piper betle). Jurnal Farmasi Tinctura 2020;2(1):34-41. doi: https://doi.org/10.35316/tinctura.v2i1.1546
Badaring DR, Sari SPM, Nurhabiba S, Wulan W, Lembang SA. Uji ekstrak daun maja (Aegle marmelos L.) terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Indonesian Journal of Fundamental Sciences 2020;6(1). doi: https://doi.org/10.26858/ijfs.v6i1.13941
Kurniawan SY, Ariami P, Rohmi. Si pinter sebagai alat penghitung koloni bakteri penunjang laboratorium mikrobiologi. J Biotek 2023;11(1):87– 97. doi: http://dx.doi.org/10.24252/jb.v11i1.35436
Mogana R, Adhikari A, Tzar MN, Ramliza R, Wiart C. Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium Miq. against bacterial clinical isolates. BMC Complement Med Ther 2020;20(55):1-11
Suryani N, Munawar F, Hajaroh S. Phytochemical screening of active secondary metabolites and antibacterial activity kaffir lime leaf (Citrus hystrix) and tumeric leaf (Curcuma longa linn.) against Escherichia coli. Al Kimiya: J Ilmu Kim Terap 2022;5(2):150–8. doi: https://doi.org/10.19109/alkimia.v5i2.11264
Sumarna S, Islam MF, Irunsah A, Sadsoeitoeboen MJ, Mandatjan KI. Phytochemicals screening and antibacterial activity of Teijsmaniadendron holrungii from West Papua. Techno J Penelitian 2023;12(1):20–7.
Sarjono PR, Putri LD, Budiarti CE, Mulyani NS, Ngadiwiyana, Ismiyarto, et al. Antioxidant and antibacterial activities of secondary metabolite endophytic bacteria from papaya leaf (Carica papaya L.). IOP Conf Ser Mater Sci Eng 2019;509:012112. doi: http://dx.doi.org/10.1088/1757-899X/509/1/012112
Nugraha SE, Achmad S, Sitompul E. Antibacterial activity of ethyl acetate fraction of passion fruit peel (Passiflora edulis Sims) on Staphylococcus aureus and Escherichia coli. IDJPCR 2019;2(1):07– 12. doi: https://doi.org/10.32734/idjpcr.v2i1.972
Prasetya R, Fatimatuzzahro N, Ermawati T, Kristina S, Prabaningrum RRH. Antibacterial activity of robusta coffee (Coffea canephora) husk extract against Enterococcus faecalis and Phorphyromonas gingivalis: in vitro study. Trends Sci 2024;21(3):1-9. doi: https://orcid.org/0000-0002-2918-2931
Rizi KS. MXene nanosheets as a novel nanomaterial with antimicrobial applications: a literature review. J Mol Struct 2022; 1262(3):132958. doi: https://doi.org/10.1016/j.molstruc.2022.132958
Nikolic P, Mudgil P. The cell wall, cell membrane and virulence factors of Staphylococcus aureus and their role in antibiotic resistance. Microorganisms 2023;11(2):259. doi: https://doi.org/10.3390/microorganisms11020259
Jubeh B, Breijyeh Z, Karaman R. Resistance of gram-positive bacteria to current antibacterial agents and overcoming approaches. Molecules 2020;25(12):2888. Doi: https://doi.org/10.3390/molecules25122888
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