Uji Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Ekstrak Etanol Daun Kaktus Pakis Giwang (Euphorbia milli) Terhadap Bakteri Pseudomonas aeruginosa

I Made Agus Sunadi Putra; Ni Putu Devi Nirmala  Putri; Puguh Santoso

doi:10.36733/usadha.v4i02.7434

Authors

I Made Agus Sunadi Putra Faculty of Pharmacy, Universitas Mahasaraswaati Denpasar
Ni Putu Devi Nirmala Putri Fakultas Farmasi Universitas Mahasaraswaati Denpasar
Puguh Santoso Faculty of Pharmacy, Universitas Mahasaraswaati Denpasar

DOI:

https://doi.org/10.36733/usadha.v4i02.7434

Keywords:

Euphorbia milli, hand sanitizer, antibacterial, Pseudomonas aeruginosa, gel

Abstract

The Covid-19 pandemic has heightened public awareness of hand hygiene thus creating a demand for safe and effective natural-based hand sanitizers. This study aimed to determine the antibacterial activity of hand sanitizer gel containing ethanol extract of Euphorbia milli leaves against Pseudomonas aeruginosa. The extract was obtained through maceration of 500 g of powdered leaves in 70% ethanol and formulated into gels with concentrations of 5%, 10%, and 15% using CMC-Na, glycerin, propylene glycol, and methyl paraben as base ingredients. Physical quality tests included organoleptic properties, homogeneity, pH, and spreadability, while antibacterial activity was assessed using the disc diffusion method on Mueller Hinton Agar. All formulations showed brown colour, homogeneous texture, and pH averaging 5.5, meeting standard requirements. The spreadability ranged between 5.0–6.0 cm, indicating good application consistency. Inhibition zone diameters against P. aeruginosa increased with extract concentration: 6.52 ± 0.26 mm (5%), 7.51 ± 0.30 mm (10%), and 8.39 ± 0.35 mm (15%), whereas the positive control (Ciprofloxacin 5 µg) produced 29.92 ± 1.06 mm and the negative control showed 0 mm. Based on inhibition zone classification, all formulations exhibited moderate antibacterial activity. In conclusion, the ethanol extract of Euphorbia milli leaves demonstrates antibacterial potential against Pseudomonas aeruginosa, with effectiveness increasing proportionally to concentration, suggesting its potential as a natural active ingredient for hand sanitizer formulations

References

[1]. Dwi Djajanti A, Muhammad Farid A, Farmasi Yamasi Makassar A, J Kesehatan Yamasi Makassar. Formulasi dan uji aktivitas sediaan gel handsanitizer minyak atsiri sereh (Cymbopogon citratus) terhadap bakteri Pseudomonas aeruginosa. J Kesehatan Yamasi Makassar. 2023;7(1):17–27.

[2]. Nikmah IS. Uji antibakteri, formulasi dan uji fisik ekstrak terpurifikasi daun katuk (Sauropus androgynus) terhadap Pseudomonas aeruginosa ATCC 27853. Semarang: Universitas Islam Sultan Agung; 2022.

[3]. Dewi HS. Uji efektivitas daya hambat antibakteri berbagai merek hand sanitizer gel terhadap Pseudomonas aeruginosa. Surakarta: Sekolah Tinggi Ilmu Kesehatan Nasional; 2021.

[4]. Indarwati SR, Salam S, Agustina R, Kunci K, Jambu Biji D. Formulasi sediaan gel ekstrak daun jambu biji (Psidium guajava L.) sebagai terapi sariawan. J Pharmasci (J Pharmacy Sci). 2023;Special Issue:18th Mulawarman Pharmaceutical Conference.

[5]. Setyawan R, et al. Formulasi, evaluasi dan uji stabilitas fisik sediaan gel antioksidan ekstrak tali putri (Cassytha filiformis L.). Bencoolen J Pharm. 2023;3(1).

[6]. Kharisma DNI, Safitri CINH, Farmasi Mitra Sehat Mandiri Sidoarjo A, Ki Hajar Dewantara J. Formulasi dan uji mutu fisik sediaan gel ekstrak bekatul (Oryza sativa L.).

[7]. Sudhan T, Varalakshmi S, Abirami T, Lenard J. Phytochemical screening, antibacterial and antioxidant activities of Euphorbia hirta crude extract. Int J Zool Invest. 2021;7(2):689–698. doi:10.33745/ijzi.2021.v07i02.052

[8]. Jayalakshmi B, Raveesha KA, Amruthesh KN. Isolation and characterization of bioactive compounds from Euphorbia cotinifolia. Futur J Pharm Sci. 2021;7(1). doi:10.1186/s43094-020-00160-9

[9]. Adjeroh LA, Nwachukwu MO, Abara PN, Nnokwe JC, Azorji JN, Osinomumu IO. Phytochemical screening and antibacterial/antifungal activities of root and shoot extracts of Euphorbia hirta (asthma weed). Int J Trop Dis Health. 2020;41(6):1–10. doi:10.9734/ijtdh/2020/v41i630281

[10]. Gerald E, Opio A. A comparative study of the effects of Jatropha multifida and Euphorbia hirta and their mixture on pathogenic growth rate. Proc Int Conf Pharmacol. 2023;63. doi:10.3390/ecp2023-14682

[11]. Arif M, et al. Green synthesis of silver nanoparticles using Euphorbia wallichii leaf extract: its antibacterial action against citrus canker causal agent and antioxidant potential. Molecules. 2022;27(11):3525. doi:10.3390/molecules27113525

[12]. Sankar HN, Shanmugam R, Anandan J. Green synthesis of Euphorbia tirucalli-mediated titanium dioxide nanoparticles against wound pathogens. Cureus. 2024. doi:10.7759/cureus.53939

[13]. Pirzada B, Tajammul A, Ahmed Z. Synergistic effect of Euphorbia milii with tannic acid as a disinfectant against Escherichia coli and Staphylococcus aureus. Proc Int Halal Sci Technol Conf. 2022;14(1):138–144. doi:10.31098/ihsatec.v14i1.495

[14]. Jayanthi S, Nuraini N, Setyoko S. Effectivity of hand sanitizer cempaka flower extract (Michelia champaca L.) on the growth of Staphylococcus aureus. Adv Soc Sci Humanit Res. 2021. doi:10.2991/assehr.k.210909.033

[15]. Wijewardhana SPR, Weerakkody NS. Potential of incorporating Alpinia malaccensis crude extract into hand sanitizers. Sri Lankan J Biol. 2024;9(1):1–8. doi:10.4038/sljb.v9i1.116