Produksi Ramah Lingkungan Granul Instan Antioksidan dari Kulit Jeruk Bali (Citrus maxima) Menggunakan Ekstraksi Berbantuan Enzim

Putu Era Sandhi Kusuma Yuda; Ni Kadek Lita; Adinda Puspita Elisabet Lian; Ni Putu Jayanti Putri; I Gusti Ayu Sri Candramukhi Devi Dasi; Intan Alfira Kolifay; Erna Cahyaningsih; Maria Malida Vernandes Sasadara

doi:10.36733/usadha.v4i3.13155

Authors

Putu Era Sandhi Kusuma Yuda Departemen Farmasi Bahan Alam, Fakultas Farmasi, Universitas Mahasaraswati Denpasar
Ni Kadek Lita
Adinda Puspita Elisabet Lian
Ni Putu Jayanti Putri
I Gusti Ayu Sri Candramukhi Devi Dasi
Intan Alfira Kolifay
Erna Cahyaningsih
Maria Malida Vernandes Sasadara

DOI:

https://doi.org/10.36733/usadha.v4i3.13155

Keywords:

Citrus maxima, instant granules, antioxidant, enzymatic extraction, eco-friendly

Abstract

Pomelo peel (Citrus maxima) is an agricultural by-product rich in bioactive compounds, particularly flavonoids and phenolic compounds, which have potential antioxidant and anti-aging properties. However, the high pectin content in citrus peels can hinder the release of these active compounds. The objective of this study was to improve the extraction efficiency of bioactive compounds from pomelo peel using an environmentally friendly Enzyme-Assisted Extraction (EAE) method employing pectinase enzymes and to formulate the extract into instant effervescent granules. The granules produced using enzymatic extraction, granules prepared without enzymes, and control granules were evaluated through sensory assessment, physical quality analysis, total phenolic content, antioxidant activity (DPPH method), and crude fiber content. The results showed that enzyme-assisted extraction granules (EEG) exhibited significantly higher free radical scavenging activity (49.57 ± 6.10%) compared to non-enzymatic granules (NEG) (28.48 ± 8.67%). EEG also demonstrated a higher total phenolic content (0.027 g/100 g) than NEG (0.017 g/100 g). In addition, EEG showed better sensory acceptance and physical quality, including a more balanced taste, a stronger citrus aroma, and higher fiber content. These findings indicate that the application of the EAE method can enhance phenolic and fiber content, physical quality, and antioxidant activity of instant pomelo peel granules, suggesting their potential development as an environmentally friendly natural antioxidant product.

References

[1]. Taylor E, Kim Y, Zhang K, Chau L, Nguyen BC, Rayalam S, et al. Antiaging Mechanism of Natural Compounds: Effects on Autophagy and Oxidative Stress. Molecules. 2022;27(14):1–24.

[2]. Dossena S, Marino A. Oxidative Stress and Antioxidants in Aging. Antioxidants. 2024;13(11):1–5.

[3]. Gupta AK, Dhua S, Sahu PP, Abate G, Mishra P, Mastinu A. Variation in phytochemical, antioxidant and volatile composition of pomelo fruit (Citrus grandis (l.) osbeck) during seasonal growth and development. Plants. 2021;10(9).

[4]. Tocmo R, Pena-Fronteras J, Calumba KF, Mendoza M, Johnson JJ. Valorization of pomelo (Citrus grandis Osbeck) peel: A review of current utilization, phytochemistry, bioactivities, and mechanisms of action. Compr Rev Food Sci Food Saf. 2020;19(4):1969–2012.

[5]. Anantami A, Wulandari S, Martono A. Ekstraksi pektin kulit jeruk bali (citrus grandis l.) Sebagai polisakarida pada edible coating. Bencoolen J Pharm. 2023;3(2):1–11.

[6]. Riyamol N, Gada Chengaiyan J, Rana SS, Ahmad F, Haque S, Capanoglu E. Recent Advances in the Extraction of Pectin from Various Sources and Industrial Applications. ACS Omega. 2023;8(49):46309–24.

[7]. Adi-Dako O, Kumadoh D, Egbi G, Okyem S, Addo PY, Nyarko A, et al. Strategies for formulation of effervescent granules of an herbal product for the management of typhoid fever. Heliyon [Internet]. 2021;7(10):e08147. Available from: https://doi.org/10.1016/j.heliyon.2021.e08147

[8]. Polo-Castellano C, Mateos RM, Visiedo F, Palma M, Fernández Barbero G, Ferreiro-González M. Optimization of the Enzymatic Extraction of Naringenin from Pink Grapefruit Pulp (Citrus × paradisi Macfad.). Agronomy. 2024;14(3).

[9]. Pratama R, Nurasih W, Asnawi A, Suhardiman A, Zaelani D, Pahlevi MR. Development and Standardization of an Effervescent Granule Formulation of Pomegranate Peel Extract with Potential Antioxidant Activity. Trop J Nat Prod Res. 2025;9(3):1112–7.

[10]. Vidana Gamage GC, Choo WS. Effect of hot water, ultrasound, microwave, and pectinase-assisted extraction of anthocyanins from black goji berry for food application. Heliyon [Internet]. 2023;9(3):e14426. Available from: https://doi.org/10.1016/j.heliyon.2023.e14426

[11]. Yilmaz E, Karatas B. Evaluation of the sensory properties, volatile aroma compounds and functional food potentials of cold-press produced mahaleb (Prunus mahaleb L.) seed oil. OCL - Oilseeds fats, Crop Lipids. 2023;30.

[12]. Mang Sung Thluai L, Titapiwatanakun V, Ruksiriwanich W, Boonpisuttinant K, Chutoprapat R. Development of Effervescent Cleansing Tablets Containing Asiatic-Acid-Loaded Solid Lipid Microparticles. Cosmetics. 2023;10(6).

[13]. Huynh MNT, Nguyen TNP, Van KC, Mai CH. Study of the extraction of bioactive compounds from pomelo (Citrus maxima) pith and applying it to drinking products. Food Res. 2025;9(3):110–9.

[14]. Singh R, Chaudhary M, Singh Chauhan E, Kumari J. International Journal of Food Science and Nutrition www.foodsciencejournal.com Effects of several processing techniques on proximate composition, antioxidant properties of citrus fruit peel and its application in value-added food products. 2022;7(4):18–22. Available from: www.foodsciencejournal.com

[15]. Ramzan K, Zehra SH, Balciunaitiene A, Viskelis P, Viskelis J. Valorization of Fruit and Vegetable Waste: An Approach to Focusing on Extraction of Natural Pigments. Foods. 2025;14(8).

[16]. Li Y, Guo L, Mao X, Ji C, Li W, Zhou Z. Changes in the nutritional, flavor, and phytochemical properties of Citrus reticulata Blanco cv. ‘Dahongpao’ whole fruits during enzymatic hydrolysis and fermentation. Front Sustain Food Syst. 2024;8(October).

[17]. Sun S, Yu Y, Jo Y, Han JH, Xue Y, Cho M, et al. Impact of extraction techniques on phytochemical composition and bioactivity of natural product mixtures. Front Pharmacol. 2025;16(July):1–14.

[18]. Dhondale MR, Thakor P, Nambiar AG, Singh M, Agrawal AK, Shastri NR, et al. Co-Crystallization Approach to Enhance the Stability of Moisture-Sensitive Drugs. Pharmaceutics. 2023;15(1).