TEST OF RESISTANCE OF ALPUKAT (Persea americana mill) FRUIT EXTRACT ON THE GROWTH OF THE Streptococcus mutans

Authors

  • Putu Rusmiany Department of Conservative, Faculty of Dentistry, Universitas Mahasaraswati Denpasar
  • Ni Nyoman Nurdeviyanti Department of Conservative, Faculty of Dentistry, Universitas Mahasaraswati Denpasar
  • Ilma Yudistian Department of Conservative, Faculty of Dentistry, Universitas Mahasaraswati Denpasar
  • Agnesia Lorenza Undergraduate Program, Faculty of Dentistry, Universitas Mahasaraswati Denpasar

DOI:

https://doi.org/10.46862/interdental.v20i1.8746

Keywords:

Avocado pulp, inhibition, streptococcus mutans

Abstract

Introduction: Streptococcus mutans  is one of the normal flora living in the oral cavity, but in excessive amounts is the main causative agent of dental caries. The active compounds of alkaloids, saponins and quinones in avocado pulp extract is an antibacterial alternative that can inhibit the growth of Streptococcus mutans  in the oral cavity. The aim of this research was to determine the inhibitory power that contain in avocado pulp against Streptococcus mutans .

Materials and Methods: This study used 4 concentrations namely 25%, 50%, 75% and 100% with each repetition 4 times. Avocado pulp extraction was carried out by maceration method using 96% ethanol solvent. Inhibition zone testing was carried out using the Kirby baurer method.

Results and Discussions: The zone of inhibition was determined using the Kruskal wallis test, which showed that there were significant differences in various concentrations against the growth of Streptococcus mutans  bacteria. Avocado fruit pulp extract is able to inhibit the growth of Streptococcus mutans  bacteria because avocado fruit pulp extract has active compounds that cause antibacterial activity. Active compounds of avocado fruit flesh that are efficacious as antibacterials are alkaloids, saponins and quinones.

Conclusion: Avocado pulp extract has been proven to have an antibacterial effect against Streptococcus mutans  with an effective concentration level of 100%. Increasing the concentration of avocado pulp extract was also shown to affect the diameter of Streptococcus mutans  inhibition zone

Downloads

Download data is not yet available.

References

Larasati R. Hubungan kebersihan mulut dengan penyakit sistemik dan usia harapan hidup. Jurnal Skala Husada 2012;9(1):97-104.

Kidd EA, Bechal SJ. Dasar-dasar karies penyakit dan penanggulangan. Jakarta: EGC; 2012.

Kusumaningsari V, Handajani J. Efek Pengunyahan Permen Karet Gula dan Xylitol terhadap Pertumbuhan Bakteri Streptococcus mutans pada Plak Gigi. Majalah Kedokteran Gigi Indonesia 2011;18(1):30-4. Doi: https://doi.org/10.22146/majkedgiind.16473

Tanzer JM, Livingston J, Thompson AM. The microbiology of primary dental caries in humans. Journal of dental education 2000;65(10):1028-37.

Forssten SD, Björklund M, Ouwehand AC. Streptococcus mutans, caries and simulation models. Nutrients 2010;2(3):290-8. Doi: https://doi.org/10.3390/nu2030290

Ernawati, Sari K. Kandungan senyawa kimia dan aktivitas antibakteri ekstrak kulit buah alpukat (Persea americana P. Mill) terhadap bakteri Vibrio alginolyticus. Jurnal Kajian Veteriner 2015;3(2):203-11. Doi: https://doi.org/10.35508/jkv.v3i2.1043

Lenny AA. Daya Hambat Ekstrak Buah Alpukat (Persea americana mill) Terhadap Pertumbuhan Staphylococcus aureus dan Staphylococcus epidermidis. Skripsi. Semarang: Universitas Muhammadiyah Semarang;2016.

Nuria MC, Faizatun A. Uji aktivitas antibakteri ekstrak etanol daun Jarak Pagar (Jatropha Curcas L) terhadap bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408. Mediagro 2009;5(2):26-37. Doi: http://dx.doi.org/10.31942/mediagro.v5i2.559

Isnarianti R, Wahyudi IA, Puspita RM. Muntingia calabura L leaves extract inhibits glucosyltransferase activity of Streptococcus mutans . Journal of Dentistry Indonesia 2013 Dec 31;20(3):59-63. Doi: http://dx.doi.org/10.14693/jdi.v20i3.195

Munawwarah L, Ramadhan AM, Ardana M. Uji Aktivitas Antibakteri Ekstrak Daun Sapat (Mitragyna speciosa Korth.) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Proceedings of Mulawarman Pharmaceuticals Conferences; 2016 Nov 1 (Vol. 4, pp. 180-186). Doi: https://doi.org/10.25026/mpc.v4i1.179

González-Lamothe R, Mitchell G, Gattuso M, Diarra MS, Malouin F, Bouarab K. Plant antimicrobial agents and their effects on plant and human pathogens. International journal of molecular sciences 2009;10(8):3400-19. Doi: https://doi.org/10.3390%2Fijms10083400

Karlina CY, Ibrahim M, Trimulyono G. Aktivitas antibakteri ekstrak herba krokot (Portulaca oleracea L.) terhadap Staphylococcus aureus dan Escherichia coli. Lentera Bio 2013;2(1):87-93.

Suratno S. Skrining Fitokimia Ekstrak Etanol Mikroalga Spirulina platensis yang Berpotensi sebagai Antibakteri: Phytochemical Screening Ethanol Extract of Spirulina platensis Microalgae which Potentially Antibacterial. Jurnal Surya Medika (JSM) 2016;1(2):26-33. Doi: https://doi.org/10.33084/jsm.v1i2.396

Al Amrie AG, Ivan I, Anam S, Pitopang R. Uji efektifitas ekstrak daun dan akar harrisonia perforata merr. terhadap pertumbuhan bakteri Vibrio cholerae. Natural Science: Journal of Science and Technology 2014;3(3):331-340. Doi: https://doi.org/10.22487/25411969.2014.v3.i3.3343

Ngajow M, Abidjulu J, Kamu VS. Pengaruh antibakteri ekstrak kulit batang matoa (Pometia pinnata) terhadap bakteri Staphylococcus aureus secara in vitro. Jurnal Mipa 2013;2(2):128-32. Doi: https://doi.org/10.35799/jm.2.2.2013.3121

Chairani A, Harfiani E. Efektivitas Getah Jarak Sebagai Antiseptik terhadap Pertumbuhan Staphylococcus aureus, Escherichia colidan Candida sp. secara In Vitro. Jurnal Kedokteran Universitas Lampung 2018;2(2):84-92.

Doi: https://doi.org/10.23960/jkunila2284-92

Sumarno. Teknik dasar pemeliharaan mikroba. Jakarta: Intan Prawira; 2000.

Fadillah H. Optimasi Sabun Cair Antibakteri Ekstrak Etanol Rimpang Jahe Merah (Zingiber Officinale Rosc. Var. Rubrum) Variasi Virgin Coconut Oil (Vco) Dan Kalium Hidroksida (Koh) Menggunakan Simplex Lattice Design. Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTAN 2014;1(1).

Downloads

Published

2024-04-21

How to Cite

1.
Rusmiany P, Nurdeviyanti NN, Yudistian I, Lorenza A. TEST OF RESISTANCE OF ALPUKAT (Persea americana mill) FRUIT EXTRACT ON THE GROWTH OF THE Streptococcus mutans. interdental [Internet]. 2024 Apr. 21 [cited 2024 Jun. 20];20(1):53-9. Available from: https://e-journal.unmas.ac.id/index.php/interdental/article/view/8746

Most read articles by the same author(s)