• Dharmmesti Anindita Wijayanti Master of Science in Clinical Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Dahlia Herawati Departement of Periodontia, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Vincensia Maria Karina Departement of Periodontia, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Kwartarini Murdiastuti epartement of Periodontia, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia



Collagen chitosan hydrogel, periodontal regenerative treatment, scaffold


Introduction: Hydrogels are considered a promising scaffold biomaterial for regenerative periodontal treatment. The development of collagen and chitosan based hydrogels can improve the final characteristics of the hydrogel.

Review: Collagen and chitosan are the two main materials used to design scaffolds for tissue engineering. Collagen is a material that is often used to develop hydrogels because it has the advantage of high biocompatibility. In the biomedical field, collagen is often used as a drug delivery system. Collagen has weak mechanical strength so other biomaterials are needed for its use. Chitosan material has high biocompatibility and a slow degradation rate. So the combination of these two materials is a strategic choice for making promising biomaterial scaffolds in periodontal regenerative treatment.

Conclusion: Chitosan collagen hydrogel is a potential scaffold biomaterial for periodontal regenerative treatment.


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Newman MG, Klokkevold PR, Satheesh Elangovan, Kapila Y. Newman and Carranza’s Clinical Periodontology and Implantology E-Book. Los Angeles: Elsevier Health Sciences; 2023.

Yamada S, Shanbhag S, Mustafa K. Scaffolds in Periodontal Regenerative Treatment. In Dental Clinics of North America 2022; 66(1):111-130. Doi:

Vaquette C, Pilipchuk SP, Bartold PM, Hutmacher DW, Giannobile WV, Ivanovski S. Tissue Engineered Constructs for Periodontal Regeneration: Current Status and Future Perspectives. Advanced Healthcare Materials2018;7(21):1800457–7.


He XT, Wu RX, Chen FM. Periodontal tissue engineering and regeneration. Elsevier eBooks. 2020;1221–49. Doi:

Drury JL, Mooney DJ. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 2003;24(24):4337–51. Doi:

Shaikh F, Neha Langade, Khan M, Sangeeta Muglikar, Nawar Zahra Ansari. Mesenchymal stem cells and tissue engineering in dentistry. IntechOpen eBooks. 2023; Doi:

Galli M, Yao Y, Giannobile WV, Wang HL. Current and future trends in periodontal tissue engineering and bone regeneration. Plastic and Aesthetic Research 2021;8:3. Doi:

Zielińska A, Karczewski J, Eder P, Kolanowski T, Szalata M, Wielgus K, et al. Scaffolds for drug delivery and tissue engineering: The role of genetics. Journal of Controlled Release 2023;359:207–23. Doi:

Zhang Y, Li Z, Guan J, Mao Y, Zhou P. Hydrogel: A potential therapeutic material for bone tissue engineering. AIP Adv 2021;11(1):010701. Doi:

Ayala-Ham A, López-Gutierrez J, Bermúdez M, Aguilar-Medina M, Sarmiento-Sánchez JI, López-Camarillo C, et al. Hydrogel-Based Scaffolds in Oral Tissue Engineering. Frontiers in Materials. 2021;8. Doi:

Choi J, Park H, Kim T, Jeong Y, Man Hwan Oh, Taeghwan Hyeon, et al. Engineered collagen hydrogels for the sustained release of biomolecules and imaging agents: promoting the growth of human gingival cells. International Journal of Nanomedicine 2014;9:5189–9. Doi:

Hamdy S, Ahmed A, Khalil, Mohammed A, Ibrahim, Ahmed Y, et al. Hydrogel scaffold in treatment of periodontal intrabony defect (review article) Section A -Research paper 5707 Eur. Chem Bull 2023:5707–10. Doi:

Sánchez-Cid P, Jiménez-Rosado M, Rubio-Valle JF, Romero A, Ostos FJ, Rafii-El-Idrissi Benhnia M, et al. Biocompatible and thermoresistant hydrogels based on collagen and chitosan. Polymers 2022;14(2):272. Doi:

Elango J. Proliferative and osteogenic supportive effect of vegf-loaded collagen-chitosan hydrogel system in bone marrow derived mesenchymal stem cells. Pharmaceutics 2023;15(4):1297–7. Doi:

Deng Z, Jin J, Wang S, Qi F, Chen X, Liu C, et al. Narrative review of the choices of stem cell sources and hydrogels for cartilage tissue engineering. Annals of Translational Medicine 2020;8(23):1598. Doi:

Ahmadi F, Z Oveisi, S Mohammadi Samani, Z Amoozgar. Chitosan based hydrogels: characteristics and pharmaceutical applications. Res Pharm Sci 2015;10(1):1–16.

Kang D, Wang W, Li Y, Ma Y, Huang Y, Wang J. Biological macromolecule hydrogel based on recombinant type i collagen/chitosan scaffold to accelerate full-thickness healing of skin wounds. Polymers 2023;15(19):3919–9. Doi:

Yang Y, Campbell Ritchie A, Everitt NM. Recombinant human collagen/chitosan-based soft hydrogels as biomaterials for soft tissue engineering. Mater Sci Eng 2021;121:111846. Doi:

Raftery RM, Woods B, Marques ALP, Moreira-Silva J, Silva TH, Cryan SA, et al. Multifunctional biomaterials from the sea: Assessing the effects of chitosan incorporation into collagen scaffolds on mechanical and biological functionality. Actabiomat 2016;43:160–9. Doi:

Donea RT, Cobzariu I, Butnaru M, Verestiuc L. methacrylated collagen/chitosan - based hydrogels as scaffolds for soft tissue engineering. Proceeding of the International Conference on e-Health and Bioengineering (EHB); 2021 Nov 18. Doi:

Eshwar S, Konuganti K, Manvi S, Bharadwaj AN, Sajjan S, Boregowda SS, et al. Evaluation of osteogenic potential of fucoidan containing chitosan hydrogel in the treatment of periodontal intra-bony defects—a randomized clinical trial. Gels. 2023;9(7):573–3. Doi:

Bhattarai N, Gunn J, Zhang M. Chitosan-based hydrogels for controlled, localized drug delivery. Advanced Drug Delivery Reviews 2010;62(1):83–99.


Chen A, Deng S, Lai J, Li J, Chen W, Swastina NV, et al. Hydrogels for oral tissue engineering: challenges and opportunities. Molecules 2023;28(9):3946–6.


Wang HL, Greenwell H, Fiorellini J, et al. Periodontal regeneration. J Periodontol 2005;76:1601–22. Doi:

Larsson L, Decker AM, Nibali L, Pilipchuk SP, Berglundh T, Giannobile WV. Regenerative

medicine for periodontal and peri-implant diseases. J Dent Res 2016;95:255–66. Doi:

Siaili M, Chatzopoulou D, Gillam DG. An overview of periodontal regenerative procedures for the general dental practitioner. The Saudi Dental Journal 2018;30(1):26–37. Doi:

Lee HS, Byun SH, Cho SW, Yang BE. Past, Present, and Future of Regeneration Therapy in Oral and Periodontal Tissue: A Review. Applied Sciences 2019;9(6):1046. Doi:

Ahmed TAE, Dare EV, Hincke M. Fibrin: A Versatile scaffold for tissue engineering applications. Tissue Engineering Part B: Reviews 2008;14(2):199–215.


Moro JDS, Barcelos RCS, Terra TG, Danesi CC. Tissue engineering perspectives in dentistry: review of the literature. RGO - Revista Gaúcha de Odontologia 2018;66(4):361–7. Doi:

Rodríguez VM, Vega RB, Ramos ZR, Saldaña KDA, Quiñones OLF. Chitosan and its potential use as a scaffold for tissue engineering in regenerative medicine. BioMed Research International 2015;2015:1–15. Doi:

Iviglia G, Kargozar S, Baino F. Biomaterials, current strategies, and novel nanotechnological approaches for periodontal regeneration. J Funct Biomater 2019;10:3. Doi:

Sharifianjazi F, Khaksar S, Esmaeilkhanian A, et al. Advancements in fabrication

and application of chitosan composites in implants and dentistry: a review.

Biomolecules 2022;12:155. Doi:

Abedi N, Rajabi N, Kharaziha M, Nejatidanesh F, Tayebi L. Layered scaffolds in periodontal regeneration. Journal of Oral Biology and Craniofacial Research 2022;12(6):782–97. Doi:

Santos MS, Alexandra, Carvalho MS. New Insights in Hydrogels for Periodontal Regeneration. J Funct Biomater 2023;14(11):545–5. Doi:

Hutomo DI, Amir L, Suniarti DF, Bachtiar EW, Soeroso Y. Hydrogel-Based Biomaterial as a Scaffold for Gingival Regeneration: A Systematic Review of In Vitro Studies. Polymers 2023;15(12):2591. Doi:

Farzin A, Bahrami N, Mohamadnia A, Mousavi S, Gholami M, Ai J, et al. Scaffolds in Dental Tissue Engineering: A Review. Arch Neurosci 2019;7(1). Doi:

Mahesh L, Kurtzman GM, Shukla S. Regeneration in periodontics: collagen-a review of its properties and applications in dentistry. Compend Contin Educ Dent 2015;36(5):358–63.

John M, Sugunan A, Aswathy S, Revu DSD. Marine based biomaterials: a marvel in periodontal regeneration – A Review. Advanced Dental Journal 2023;5(1):24–34. Doi:

Kean T, Thanou M. Biodegradation, biodistribution and toxicity of chitosan. Adv Drug Deliv Rev 2010;62(1):3–11. Doi:

Si J, Yang Y, Xing X, Yang F, Shan P. Controlled degradable chitosan/collagen composite scaffolds for application in nerve tissue regeneration. Polymer Degradation and Stability 2019;166:73–85. Doi:

Baldrick P. The safety of chitosan as a pharmaceutical excipient. Regulatory Toxicology and Pharmacology 2010;56(3):290–9. Doi:

Donnaloja F, Jacchetti E, Soncini M, Raimondi MT. Natural and Synthetic Polymers for Bone Scaffolds Optimization. Polymers 2020;12(4):905. Doi:

Gorgieva S, Kokol V. Collagen- vs. gelatine-based biomaterials and their biocompatibility: review and perspectives. Biomaterials Applications for Nanomedicine.InTech;2011.p.17 Doi:

Rosdiani AF, Widiyanti P, Rudyarjo DI. Synthesis and characterization biocomposite collagen-chitosan-glycerol as scaffold for gingival recession therapy. J Int Dent Med Res 2017;10(1):118–22.

Perez-Puyana V, Rubio-Valle JF, Jiménez-Rosado M, Guerrero A, Romero A. Alternative processing methods of hybrid porous scaffolds based on gelatin and chitosan. J Mech Behav Biomed Mater 2020;102:103472. Doi:

Khan A, Alamry KA. Recent advances of emerging green chitosan-based biomaterials with potential biomedical applications: A review. Carbohydr Res 2021;506:108368. Doi:

Gull N, Khan SM, Butt OM, Islam A, Shah A, Jabeen S, et al. Inflammation targeted chitosan-based hydrogel for controlled release of diclofenac sodium. Int J Biol Macromol 2020;162:175–87. Doi:

Tan W, Krishnaraj R, Desai TA. Evaluation of Nanostructured Composite Collagen–Chitosan Matrices for Tissue Engineering. Tissue Eng 2001;7(2):203–10. Doi:

Indrani DJ, Lukitowati F, Yulizar Y. Preparation of chitosan/collagen blend membranes for wound dressing: a study on ftir spectroscopy and mechanical properties. IOP Conference Series: Materials Science and Engineering 2017;202(1):012020–0. Doi:

Sidharta K, Suryono, Murdiastuti K, Pritia MA. Effect of collagen chitosan hydrogel with injectable platelet-rich fibrin on alkaline phosphatase activity and calcium deposition an in vitro study on osteoblast-like cell line MG63. Research Square 2023;1:1-12. Doi:

Pritia MA, Fatimah N, Karina VM, Murdiastuti K. Combination effect of collagen-chitosan hydrogel and injectable platelet-rich fibrin fractionation on osteoblast migration and proliferation. European Journal of Dental and Oral Health 2023;4(3):22–7. Doi:

Anindyajati TP, Lastianny SP, Yogianti F, Murdiastuti K. Effect of collagen-chitosan hydrogel formula combined with platelet-rich plasma (A study of ph, viscocity, and swelling test). MKGI 2021;6(3):123. Doi:

Anindyajati TP, Lastianny SP, Martien R, Murdiastuti K. The Effect of cytotoxicity of collagen-chitosan hydrogel on platelets-rich plasma various formulation for human primary fibroblast. Mal J Med Health Sci 2020;16(SUPP15):2636–9346.

Anindyajati TP. Pengaruh open flap debridement dengan penambahan hidrogel kolagen kitosan platelet-rich plasma pada perawatan poket infraboni. Tesis. Yogyakarta: Pascasarjana Universitas Gadjah Mada; 2020.




How to Cite

Wijayanti DA, Herawati D, Karina VM, Murdiastuti K. CHITOSAN COLLAGEN HYDROGEL: A POTENTIAL SCAFFOLD BIOMATERIAL FOR PERIODONTAL REGENERATIVE TREATMENT. interdental [Internet]. 2024 Apr. 21 [cited 2024 Jun. 20];20(1):124-32. Available from: