• James Hartono Santoso Undergraduate Program, Faculty of Dentistry, Trisaki University, Jakarta, Indonesia
  • Anastasia Elsa Prahasti Department of Conservative Dentistry, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia
  • Elline Department of Conservative Dentistry, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia




Dental enamel, dental enamel hardness, dental erosion, hardness number, hydroxyapatite paste


Introduction: Dental erosion can affect the dental demineralization due to the pH level that is below the critical pH of 5,5 resulting a decrease in dental enamel hardness. Remineralization is needed to bring back the mineral loss. Hydroxyapatite is similar to inorganic components in dental enamel dan have biocompatible properties. Hydroxyapatite with 4 wt% concentration has a Ca/P molar ratio close to 1,67, which is similar to the human enamel and can regenerate enamel. This study is aimed to analyze the effects of 4 wt% hydroxyapatite paste on dental enamel hardness for 7 and 14 days.

Material and Methods: 20 maxillary premolars were divided into 4 groups. Group A1 were applied with 4 wt% hydroxyapatite paste for 30 minutes each day for 7 days, group A2 were applied with 4 wt% hydroxyapatite paste for 30 minutes each day for 14 days, group B1 were immersed with artificial saliva for 7 days, and group B2 were immersed with artificial saliva for 14 days. All groups were immersed with artificial saliva and was incubated for 24 hours at 37°C. Hardness measurements were carried out at the start, after erosion, and at the end of the experiment. Dental enamel hardness was measured with Vickers Hardness Tester (Shimadzu HMV G-21 Series). Dental enamel hardness data were analysed with GLM Repeated Measure ANOVA test (p<0,05) and One-way ANOVA test (p<0,05).

Results and Discussions: The result showed dental enamel hardness number after 4 wt% hydroxyapatite paste application showed significant difference than untreated group, which was immersed in artificial saliva. Dental enamel hardness number after 4 wt% hydroxyapatite paste application for 14 days showed significant difference than 7 days.

Conclusion: It can be concluded that 4 wt% hydroxyapatite paste application for 14 days resulted in an increase of dental enamel hardness as in normal condition of dental enamel before erosion


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Schlueter N, Luka B. Erosive tooth wear - A review on global prevalence and on its prevalence in risk groups. Br Dent J 2018; 224(5):364–70. Doi: https://doi.org/10.1038/sj.bdj.2018.167

Paryag A, Rafeek R. Dental erosion and medical conditions an overview of aetiology, diagnosis and management. West Indian Med J 2014; 63(5):499– 502. Doi: https://doi.org/10.7727%2Fwimj.2013.140

Garg N, Garg A. Textbook of operative dentistry. Fourth ed. New Delhi: Jaypee Brothers; 2020. p. 388.

Mount GJ, Hume WR, Ngo HC, Wolff MS. Preservation and restoration of tooth structure. Third ed. New Jersey: Wiley Blackwell; 2016. p.14.

Gaalová P, Galusková D, Kováč J, Kováč D, Galusek D. Corrosion in acidic beverages and recovery of microhardness of human teeth enamel. Ceramics – Silikaty 2016; 60(2):105– 14. Doi: http://dx.doi.org/10.13168/cs.2016.0016

Irianti AN, Kuswandari S, Santoso AS. Effect of formula milk on the roughness and hardness of tooth enamel. Dent J 2021;54(2):78–81. Doi: https://doi.org/10.20473/j.djmkg.v54.i2.p78-81

Arifa MK, Ephraim R, Rajamani T. Recent advances in dental hard tissue remineralization: A review of literature. Int J Clin Pediatr Dent 2019; 12(2):139– 44. Doi: https://doi.org/10.5005%2Fjp-journals-10005-1603

Bajaj M, Poornima P, Praveen S, Nagaveni NB, Roopa KB, Neena IE, et al. Comparison of CPP-ACP, Tri- Calcium phosphate and hydroxyapatite on remineralization of artificial caries like lesions on primary enamel: An in vitro study. J Clin Pediatr Dent 2016; 40(5):404–9. Doi: https://doi.org/10.17796/1053-4628-40.5.404

Chen L, Al-Bayatee S, Khurshid Z, Shavandi A, Brunton P, Ratnayake J. Hydroxyapatite in oral care products: A review. Materials (Basel) 2021; 14(17):4865. Doi: https://doi.org/10.3390%2Fma14174865

Utama J, Elline E, Subrata A, Prahasti AE, Azman SA. Cytotoxicity test of chicken eggshell-based hydroxyapatite on human dental pulp cells. Sci Dent J 2023; 7:22–5. Doi:10.4103/SDJ.SDJ_6_23

Elline E, Ismiyatin K, Indah Budhy T, Bhardwaj A. The potential of egg shell hydroxyapatite, collagen, and EGCG (HAp-Col-EGCG) scaffold as a pulp regeneration material. Saudi Dent J 2022; 34(8):715–22. Doi: https://doi.org/10.1016%2Fj.sdentj.2022.10.004

Molaasadolah F, Eskandarion S, Ehsani A, Sanginan M. In vitro evaluation of enamel micro hardness after application of two types of fluoride varnish. J Clin Diagn Res 2017; 11(8):ZC64–6. Doi: https://doi.org/10.7860%2FJCDR%2F2017%2F30121.10412

Mona D, Rifani A. Analisis perbedaan kekerasan permukaan enamel gigi antara perendaman dalam jus mangga dan jus jambu biji secara in vitro. Jurnal Human Care 2022; 6(1):113–9. Doi: http://dx.doi.org/10.32883/hcj.v6i1.1087

Setyawati A, Silviana F. Pengaruh Pasta Cangkang Telur Ayam Negeri Terhadap Email Gigi. DJKG 2019; 13(2):24–30.

Devitasari SP, Hudiyati M, Anastasia D. Effect of Hydroxyapatite From Waste Of Tilapia Bone (Oreochromis niloticus) On The Surface Hardness Of Enamel. J Physc.: Conf. Ser 2019; 1246(012009):1–7. Doi: http://dx.doi.org/10.1088/1742-6596/1246/1/012009

Dehtjars J, Bistrovs V, Bistrova N, Karlov A, Meissner C, Kataševs A, Paramonova E, Poļaka N. Size depended electrical properties of hydroxyapatite nanoparticles. In:IFMBE Proceedings. Vol.25/8: World Congress on Medical Physics and Biomedical Engineering, Germany, Munich, 7-12 September, 2009. Berlin: Springer Berlin Heidelberg, 2010, pp.230-232. ISBN 9783642038860.

Fabritius-Vilpoux K, Enax J, Herbig M, Raabe D, Fabritius HO. Quantitative affinity parameters of synthetic hydroxyapatite and enamel surfaces in vitro. Bioinsp, Biomim and Nanobiomaterials 2019; 8(2):141–53. Doi: http://dx.doi.org/10.1680/jbibn.18.00035

Fontaine A La, Zavgorodniy A, Liu H, Zheng R, Swain M, Cairney J. Atomic- scale compositional mapping reveals Mg-rich amorphous calcium phosphate in human dental enamel. Sci Adv 2016; 2(9):e1601145. Doi: https://doi.org/10.1126/sciadv.1601145

Meyer F, Amaechi BT, Fabritius HO, Enax J. Overview of calcium phosphates used in biomimetic oral care. Open Dent J 2018; 12(1):406–23. Doi: https://doi.org/10.2174%2F1874210601812010406

Enax J, Fabritius HO, Fabritius-Vilpoux K, Amaechi BT, Meyer F. Modes of action and clinical efficacy of particulate hydroxyapatite in preventive oral health care − state of the art. Open Dent J 2019; 13(1):274–87. Doi: http://dx.doi.org/10.2174/1874210601913010274

Meyer F, Enax J, Amaechi BT, Limeback H, Fabritius HO, Ganss B, et al. Hydroxyapatite as remineralization agent for children’s dental care. Front. Dent. Med 2022; 3:3859560. Doi: https://doi.org/10.3389/fdmed.2022.859560

Vacharangkura A, Kunawarote S. Effects of experimental nano- hydroxyapatite pastes on remineralization of early demineralized enamel. RSU International Research Conference 2021; 109–21.

Clift F. Artificial methods for the remineralization of hydroxyapatite in enamel. Mater Today Chem 2021; 21:100498. Doi: http://dx.doi.org/10.1016/j.mtchem.2021.100498

Ilyasa Da, Putri LSDE, Joelijanto R. Pemanfaatan ekstrak limbah biji buah alpukat (Persea americana) sebagai inhibitor korosi pada kawat ortodonti berbahan dasar nikel titanium: studi eksperimental laboratoris. J Ked Gi 2023; 35(2):172–8. Doi: 10.24198/jkg.v35i2.47779




How to Cite

Santoso JH, Prahasti AE, Elline. EFFECT OF 4 wt% HYDROXYAPATITE PASTA ON DENTAL EMAIL HARDNESS. interdental [Internet]. 2024 Apr. 21 [cited 2024 Jun. 20];20(1):46-52. Available from: https://e-journal.unmas.ac.id/index.php/interdental/article/view/8580