LD50 ACUTE TOXICITY TEST OF GREEN GRASS JELLY (Cyclea barbata Miers) LEAF EXTRACT AGAINST MICE (Mus musculus L.)

UJI TOKSISITAS AKUT LD50 EKSTRAK DAUN CINCAU HIJAU (Cyclea barbata Miers) TERHADAP MENCIT (Mus musculus L.)

Abstract

Introduction: Green grass jelly leaves (Cyclea barbata Miers) are well known by the public as food to reduce fever (fever), nausea, stomach ulcers, coughs and reduce high blood pressure, antioxidants, anti-inflammatories, and as analgesics, so safety needs to be controlled. Acute Toxicity Test is a test used to detect the toxic effect of a substance on a biological system and to obtain typical response data from test preparations, Lethal Dose 50 and toxicity classification. Research Objectives: To determine the safety of green grass jelly leaf extract (Cyclea barbata Miers) against mice (Mus musculus L.) by LD50 acute toxicity test. Research Methods: In the acute toxicity test using the Thomson-Weil calculation method and mice as experimental animals as many as 25 mice (Mus musculus). The mice were divided into 5 treatment groups with the main test series group I distilled water as the control, group II was given the test extract at a dose of 200 mg/kg, group III was given the test extract at a dose of 400 mg/kg, group IV was given the test extract at a dose of 800 mg/kg, and Group V was given the test extract at a dose of 1600 mg/kgBW with one oral administration and observed the number of deaths in each test animal after 24 hours to 14 days. Research Results: The results of the acute toxicity test showed that the LD50 value of green grass jelly leaf extract was 0.6166 gr/kg BW (moderately toxic). For acute toxicity, the weights of mice were analyzed using the ANOVA and LSD tests and for allergy testing using the Wilcoxon signed ranks test. Conclusion: the results of the analysis show that there is a significant difference at the test level of 0.05 (???? < 0.05).