Chitosan Gingival Mask Based on Guava Leaf Extract To Prevent Smoker’s Melanosis

Authors

  • Rezmelia Sari Periodontia Department, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia
  • Lutfia Nur Sabrina Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia
  • Efrin Qorina Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia
  • Aulia Nathaniela Farin Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia

DOI:

https://doi.org/10.46862/interdental.v22i2.12411

Keywords:

Esthetic, smoker’s melanosis, guava leaf, mucoadhesive mask, chitosan

Abstract

Introduction: Smoker’s melanosis is a common periodontal condition characterized by gingival hyperpigmentation due to excessive tyrosinase activity, which affects aesthetics. Guava leaves contain flavonoids, vitamin C, and coumarin, which have been shown to inhibit pigmentation in the skin and hair. Mucoadhesive films made from chitosan shrimp shell waste are biodegradable and have been proven to be effective as drug delivery systems. This literature review aims to establish a theoretical foundation for the potential combination of guava leaf extract and shrimp waste chitosan as a gingival mask for preventing smoker's melanosis. Literature was systematically compiled from Scopus, ScienceDirect, PubMed, and SAGE, from 2014 to 2024. Inclusion and exclusion criteria were used to formulate a theoretical foundation.

Review: The literature shows that flavonoids in guava leaves inhibit ORAI1 calcium channels, vitamin C blocks copper ion bonds, and coumarin prevents the formation of ROS. These mechanisms play crucial roles in inhibiting tyrosinase. Chitosan Mucoadhesive patches with vitamin C solvent enhance the biocompatibility and effectiveness of active substance delivery.

Conclusion: The chitosan–guava leaf gingival mask holds potential for preventing smoker’s melanosis through targeted tyrosinase inhibition. Further research is required to optimize the potential of combining guava leaf extract and shrimp waste chitosan for use as a gingival mask.

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References

1. Mahayni M, Kujan O, Hamadah O. Aesthetic gingival melanin pigmentation treatment in smokers and non-smokers: a comparison study using Nd: YAG laser and ceramic bur. J Pers Med. 2023;13(7):1034. DOI:10.3390/jpm13071034.

2. Bilal K, Mehboob F, Akhtar N, Mirza IA, Okla MK, Dar MJ, et al. Wound healing, antioxidant and antibacterial activities of polyphenols of Psidium guajava L. leaf. South African J Bot. 2024;165(5):538–51. DOI:10.1016/j.sajb.2023.12.026.

3. Park H, Kim B, Kang Y, Kim W. Study on chemical composition and biological activity of Psidium guajava leaf extracts. Curr Issues Mol Biol. 2024;46(3):2133–43. DOI:10.3390/cimb46030137.

4. National Center for Biotechnology Information. PubChem Taxonomy Summary for Taxonomy 120290, Psidium guajava (guava). Available in https://pubchem.ncbi.nlm.nih.gov/taxonomy/Psidium-guajava. Accessed on October, 2024.25.

5. Rehman MAU, Khan I. Medicinal and pharmacological potential of Guava leaf powder, A Review. Int J Nat Med Heal Sci. 2022;1(2):55–60. DOI:10.52461/ijnms.v1i2.834.

6. Bakshi PS, Selvakumar D, Kadirvelu K, Kumar NS. Chitosan as an environment-friendly biomaterial - a review on recent modifications and applications. Int J Biol Macromol. 2020;150:1072–1083. DOI:10.1016/j.ijbiomac.2019.10.113.

7. Lingait D, Rahagude R, Gaharwar SS, Das RS, Verma, MG, Srivastava N, et al. A review on versatile applications of biomaterial/polycationic chitosan: An insight into the structure-property relationship. Int J Biol Macromol. 2024;257(Pt2):128676. DOI:10.1016/j.ijbiomac.2023.128676.

8. Abdel Moneim RA, El Deeb M, Rabea AA. Gingival pigmentation (cause, treatment and histological preview). Futur Dent J. 2017;3(1):1–7. DOI:10.1016/j.fdj.2017.04.002.

9. Komori T, Ono M, Hara ES, Ueda J, Nguyen HTT, Nguyen HT, et al. Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium. Sci Rep. 2018; 8(1):2612. DOI:10.1038/s41598-018-21000-0.

10. Lu L, Zhang X, Kang Y, Xiong Z, Zhang K, Xu X, et al. Novel coumarin derivatives as potential tyrosinase inhibitors: Synthesis, binding analysis and biological evaluation. Arab J Chem. 2023;16(6):104724. DOI:10.1016/j.arabjc.2023.104724.

11. Sam M, Raj CA, Santhi VP, Amalraj S, Murugan R, Gangapriya P, et al. A comparative analysis of leaf essential oil profile, in vitro biological properties and in silico studies of four Indian Guava (Psidium guajava L.) cultivars, a promising source of functional food. South African J Bot. 2023;153:357–69. DOI:10.1016/j.sajb.2023.01.005.

12. Feng X-H, Wang Z-H, Meng D-L, Li X. Cytotoxic and antioxidant constituents from the leaf of Psidium guajava. Bioorg Med Chem Lett. 2015;25(10):21938. DOI:10.1016/j.bmcl.2015.03.058.

13. Paris AL, Caridade S, Colomb E, Bellina M, Boucard E, Verrir B, et al. Sublingual protein delivery by a mucoadhesive patch made of natural polymers. Acta Biomater. 2021;128:222–35.DOI:10.1016/j.actbio.2021.04.024.

14. Yussif NM, Abdel Rahman AR, ElBarbary A. Minimally invasive non-surgical locally injected vitamin C versus the conventional surgical depigmentation in treatment of gingival hyperpigmentation of the anterior esthetic zone: A prospective comparative study. Clin Nutr Exp. 2019;24(2019):54–65. DOI:10.1016/j.yclnex.2018.12.003.

15. Ghazi S. Do the polyphenolic compounds from natural products can protect the skin from ultraviolet rays? Results Chem. 2022;4(3S1):100428. DOI:10.1016/j.rechem.2022.100428.

16. Gunia-Krzyżak A, Popiół J, Słoczyńska K, Żelaszczyk D, Koczurkiewicz-Adamczyk P, Wójcik-Pszczoła K, et al. Discovery of (E)-3-(4-chlorophenyl)-N-(5-hydroxypentyl)acrylamide among N-substituted cinnamamide derivatives as a novel cosmetic ingredient for hyperpigmentation. Bioorg Chem. 2024;150:107533. DOI:10.1016/j.bioorg.2024.107533.

17. Lee D-U, Weon KY, Nam D-Y, Nam JH, Kim WK. Skin protective effect of guava leaf against UV-induced melanogenesis via inhibition of ORAI1 channel and tyrosinase activity. Exp Dermatol. 2016;25(12):977–82. DOI:10.1111/exd.13151.

18. Ha AC, Le TM. Improvement of anti-tyrosinase activity in potential skin whitening products by combining herbal extracts and reducing their tannin content by collagen fibre adsorption. South African J Bot. 2023;155:118–26. DOI:10.1016/j.sajb.2023.02.020.

19. Khonthun C, Khoothiam K, Chumphukam O, Thongboontho R, Oonlao P, Nuntaboon P, et al. Screening and characterization of antioxidant, anti-aging, and anti-microbial activity of herbal extracts. Funct Foods Heal Dis. 2023;13(2):52–68. DOI:10.31989/ffhd.v13i2.1070.

20. Sutthisawatkul P, Piyanaetitham P, Chareonviriyaphap T, Leepasert T, Taengphan W, Karpkird T. Microemulsion containing guava leaf essential oil: Enhanced anti- inflammatory, anti-oxidation, anti-tyrosinase activities and skin permeation. J Drug Deliv Sci Technol. 2024;95:105536. DOI:10.2139/ssrn.4681356.

21. Aranaz I, et al. Chitosan: An overview of its properties and applications. Polym. 2021;13(19):3256. DOI:10.3390/polym13193256.

22. Widodorini T, Faurin AAF. Anti-bacterial effect of chitosan mucoadhesive patch combined with Rambutan peel extract (Nephelium lappaceum) on decreasing the number of Porphyromonas gingivalis. Malaysian J Med Heal Sci. 2023;19(SUPP5):77–80. Available from:https://medic.upm.edu.my/upload/dokumen/2023051510123513_2022_0887.pdf

23. Widodorini T, Hanif RS. Rambutan peel (Nephelium lappaceum L.) extract in chitosan mucoadhesive patch to decrease the number of neutrophil cells after Porphyromonas gingivalis induction. Malaysian J Med Heal Sci. 2023;19(SUPP5):71–6. Available from:https://medic.upm.edu.my/upload/dokumen/2023051510114812_2022_0886.pdf

24. Chamsai B, Opanasopit P, Samprasit W. Fast disintegrating dosage forms of mucoadhesive-based nanoparticles for oral insulin delivery: Optimization to in vivo evaluation. Int J Pharm. 2023;647:123513. DOI:10.1016/j.ijpharm.2023.123513.

25. Taghizadeh F, Mehryab F, Mortazavi SA, Rabbani S, Haeri A. Thiolated chitosan hydrogel-embedded niosomes: A promising crocin delivery system toward the management of aphthous stomatitis. Carbohydr Polym. 2023;318:121068. DOI:10.1016/j.carbpol.2023.121068.

26. Sahatsapan N, et al. Feasibility of mucoadhesive chitosan maleimide-coated liposomes for improved buccal delivery of a protein drug. J Drug Deliv Sci Technol. 2022; 69(103173). DOI:10.1016/j.jddst.2022.103173.

27. Freag MS, Saleh WM, Abdallah OY. Exploiting polymer blending approach for fabrication of buccal chitosan-based composite sponges with augmented mucoadhesive characteristics. Eur J Pharm Sci. 2018;120:10–9. DOI:10.1016/j.ejps.2018.04.041.

28. Hanif S, Sarfraz RM, Syed MA, Mahmood A, Hussain Z. Smart mucoadhesive buccal chitosan/ HPMC scaffold for sore throat: In vitro, ex vivo and pharmacokinetic profiling in humans. J Drug Deliv Sci Technol. 2022;71(1):103271. DOI:10.1016/j.jddst.2022.103271.

29. Liao Q, Lin L, Tang R, XU Z, Kong S, Lv D, et al. Preparation and characterization of mussel-inspired chitosan/polydopamine films and their feasibility for oral mucosa application. Int J Biol Macromol. 2024;279(Pt 3):135179. DOI:10.1016/j.ijbiomac.2024.135179.

30. Tejada G, Piccirilli GN, Sortino M, Salomón CJ, Lamas MC, Leonardi D. Formulation and in-vitro efficacy of antifungal mucoadhesive polymeric matrices for the delivery of miconazole nitrate. Mater Sci Eng C. 2017;79:140–50. DOI:10.1016/j.msec.2017.05.034.

31. Wang X, Yuan Z, Tao A, Wang P, Xie W, Yang S, et al. Hydrogel-based patient- friendly photodynamic therapy of oral potentially malignant disorders. Biomaterials. 2022; 281:121377. DOI:10.1016/j.biomaterials.2022.121377.

32. Nour EM, El-Habashy SE, Shehat MG, Essawy MM, El-Moslemany RM, Khalafallah NM. Atorvastatin liposomes in a 3D-printed polymer film: a repurposing approach for local treatment of oral candidiasis. Drug Deliv Transl Res. 2023;13(11):2847–68. DOI:10.1007/s13346-023-01353-4.

33. Elsharawy AM, Shukr MH, Elshafeey AH. Optimization and in vivo evaluation of duloxetine hydrochloride buccoadhesive lyophilized tablets. J Drug Deliv Sci Technol. 2019;52(1):282–91. DOI:10.1016/j.jddst.2019.04.033.

34. Hazzah HA, Farid RM, Nasra MMA, El-Massik MA, Abdallah OY. Lyophilized sponges loaded with curcumin solid lipid nanoparticles for buccal delivery: Development and characterization. Int J Pharm. 2015;492(1–2):248–57. DOI:10.1016/j.ijpharm.2015.06.022.

35. Colley HE, Said Z, Santocildes-Romero ME, Baker SR, Apice KD, Hansen J, et al. Pre-clinical evaluation of novel mucoadhesive bilayer patches for local delivery of clobetasol-17-propionate to the oral mucosa. Biomaterials. 2018;178:134–46. DOI:10.1016/j.biomaterials.2018.06.009.

36. Khajuria DK, Patil ON, Karasik D, Razdan R. Development and evaluation of novel biodegradable chitosan-based metformin intrapocket dental film for the management of periodontitis and alveolar bone loss in a rat model. Arch Oral Biol. 2018;85:120–9. DOI:10.1016/j.archoralbio.2017.10.009.

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Published

2026-08-12

How to Cite

1.
Sari R, Sabrina LN, Qorina E, Farin AN. Chitosan Gingival Mask Based on Guava Leaf Extract To Prevent Smoker’s Melanosis . interdental [Internet]. 2026 Aug. 12 [cited 2026 Aug. 13];22(2):371-8. Available from: https://e-journal.unmas.ac.id/index.php/interdental/article/view/12411

Issue

Vol. 22 No. 2 (2026): Interdental Jurnal Kedokteran Gigi (IJKG)

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Articles

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Copyright (c) 2026 Rezmelia Sari, Lutfia Nur Sabrina; Efrin Qorina, Aulia Nathaniela Farin

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