Comparative Effects of 50% and 100% Pleurotus ostreatus Gel on TNF-α in Traumatic Ulcer Healing in Wistar Rats
DOI:
https://doi.org/10.46862/interdental.v22i1.12362Keywords:
Male Wistar rat, Tumor Necrosis Factor-Alpha, traumatic ulcer, white oyster mushroomAbstract
Introduction: White oyster mushroom (Pleurotus ostreatus) is a natural ingredient with diverse benefits, including its ability to heal traumatic ulcers. In the healing process, TNF-α acts as an inflammatory mediator that promotes epithelial growth and neovascularization.
Material and Methods: This study was an in vivo laboratory experimental study using a post-test only control group approach, involving 32 samples randomly divided into eight treatment groups (n=4) based on variations in gel concentration (50%, 100%, negative control, and positive control) and observation time (days 3 and 7). TNF-α expression measurements were performed using an ELISA kit. Research data were analysed using the parametric One-Way ANOVA test, the non-parametric Kruskal-Wallis and Mann-Whitney tests, followed by the Tukey Post-Hoc test.
Results and Discussions: Statistical analysis results showed a p-value of 0.000 <0.05 between treatment groups. Tumor Necrosis Factor-Alpha (TNF-α) expression decreased significantly in the groups given 50% and 100% white oyster mushroom extract gel, with the lowest average found at a concentration of 50% on both days 3 and 7, namely 231.33 and 195.33, thus demonstrating more optimal effectiveness at that concentration.
Conclusion: White oyster mushroom (Pleurotus ostreatus) extract gel at a concentration of 50% was more effective than a concentration of 100% in reducing TNF-α expression in the healing of traumatic ulcers in male wistar rats. Further research with a concentration range of 50-100% and other immunological parameters is required for further confirmation.
Downloads
References
1. Prehananto H, Purnamasari VD, Yuliastri N. Uji aktivitas ekstrak daun kemangi dalam meningkatkan jumlah fibroblas pada traumatik ulser. Bhakta Dent J 2023; 1(1): 1–8.
2. Regezi JA, Sciubba J, Jordan RCK. Oral Pathology: Clinical Pathologic Correlations. Elsevier Health Sciences; 2016.
3. Herawati E, Nur’aeny N. Etiologi, distribusi lokasi, dan terapi ulser traumatik pada pasien di Rumah Sakit Gigi dan Mulut Universitas Padjadjaran. B-Dent J Kedokt Gigi Univ Baiturrahmah 2021; 8(3): 313–9. Doi: 10.33854/jbd.v8i3.1022
4. Kristyanti Y, Lutsina NW. Penyuluhan dan edukasi tentang pemanfaatan tanaman herbal untuk kesehatan gigi dan gusi di jemaat GKII Filadelfia Family Alak. Maj Cendekia Mengabdi 2023; 1(4): 288–91. Doi: 10.63004/mcm.v1i4.266.
5. Jantaramanant P, Sermwittayawong D, Noipha K, Hutadilok-Towatana N, Wititsuwannakul R. [Beta]-glucan-containing polysaccharide extract from the grey oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] stimulates glucose uptake by the L6 myotubes. Int Food Res J. 2014;21(2):779.
6. Andrini N. Pengaruh beta-glukan dari ekstrak ragi roti (Saccharomyces cerevisiae) terhadap penyembuhan luka sayat pada mencit Wistar jantan. J Ilm Simantek 2021; 5(4): 35–44.
7. Wibowo E, Dharmana E, Maharani N, Budijitno S. Pengaruh kombinasi suplementasi omega 3 dengan kalori restriksi terhadap penyembuhan luka bakar pada tikus Sprague Dawley kajian pada: Tumor nekrosis faktor-alfa dan perbaikan luas luka bakar. Medica Hosp J Clin Med. 2020; 7(1): 75–81. Doi: 10.36408/mhjcm.v7i1.432.
8. Sabban If, Wahyuni In. Efektifitas Ekstrak Jamur Tiram (Pleurotus Ostreatus) Terhadap Penyembuhan Ulkus Traumatik Pada Tikus (Rattus Novergicus Berkenhout 1769). In: Prosiding Sintesis (Seminar Nasional Sains, Teknologi dan Analisis). 2018.
9. AlTasyah MH, Winahyu DA, Ulfa AM. Uji Toksisitas Ekstrak Etanol Jamur Tiram Putih (Pleurotus ostreatus) Dengan Metode BSLT (Brine Shrimp Lethality Test). JFM (Jurnal Farm Malahayati) 2022; 5(1): 98–107. Doi: 10.33024/jfm.v5i1.5707
10. Savira A, Mujayanto R, Amurwaningsih M. Bay leaf (Syzygium Polyanthum) extract gel effect on Tnf-Α expression in traumatic ulcers healing process. ODONTO Dent J 2020; 7(1): 25. Doi: 10.30659/odj.7.1.25-30
11. Zahid S, Chinnam S, Ahmed A, Masaud SM, Khurshid B, Farman S, et al. RSM-based optimization of dose response and antibacterial potential of Cannabis sativa (L.) leaves using computational analysis. Dose Response 2025; 23(4): 15593258251404068. Doi: 10.1177/15593258251404067
12. Schober P, Boer C, Schwarte LA. Correlation coefficients: appropriate use and interpretation. Anesth Analg. 2018; 126(5): 1763–8. Doi: 10.1213/ANE.0000000000002864
13. Sharifi-Rad J, Quispe C, Herrera-Bravo J, Martorell M, Sharopov F, Tumer TB, Taheri Y MN. Mushroom polysaccharides as potential anticancer agents: A mechanistic review. Int J Mol Sci. 2020; 21(2): 635.
14. Rahmadani N, Wahyukundari MA, Harmono H. Efektivitas gel ekstrak biji kopi robusta (Coffea canephora) terhadap peningkatan jumlah fibroblas pada penyembuhan luka pasca gingivektomi. STOMATOGNATIC-Jurnal Kedokt Gigi 2022; 19(1): 13–8. Doi: 10.19184/stoma.v19i1.30694
15. Luo Z, Ming L, Ji J, Huang Q. U-Shaped dose-dependent modulation of macrophages by ginkgolide b mitigates systemic inflammation in septic rats. Dose-Response. 2025; 23(4): 15593258251406112. Doi: 10.1177/15593258251406112
16. Mendrofa AN, Mulawarmanti D. Ekstrak daun mangrove (A. marina) mempercepat kesembuhan ulkus traumatikus (Extract of mangrove leaf (A. marina) accelerates the healing of traumatic ulcer). J Dentomaxillofacial Sci. 2015; 14(1): 11–4.
17. García-Mediavilla MV, Crespo I, Collado PS, Esteller A, Sánchez-Campos S, Tuñón MJ G-GJ. Edible and medicinal mushrooms reduce inflammation in obese Wistar rats. Int J Mol Sci. 2023; 24(9): 7893.
18. Ningrum IS. Pengaruh ekstrak daun binahong (Anredera cordifolia (Ten.) Steenis) terhadap ekspresi tumor necrosis factor-α (tnf-α) pada ulkus mulut (penelitian in vivo). Karya Tulis Ilmiah. Semarang: Universitas Islam Sultan Agung; 2021.
19. Cladis DP, Weaver CM, Ferruzzi MG. (Poly) Phenol toxicity in vivo following oral administration: A targeted narrative review of (poly) phenols from green tea, grape, and anthocyanin‐rich extracts. Phyther Res. 2022; 36(1): 323–35. Doi: 10.1002/ptr.7323
20. Siddique MF, Selim MYE, Ghannam MT, Huh J, Husain A. Enhancing the stability and rheological performance of date seed powder biomass-based slurry fuels: Influence of dispersants and temperature effects. Energy Convers Manag. 2025; 342: 120126. Doi: 10.1016/j.enconman.2025.120126
21. Karthikeyan M, Mehta A, Kumar SS, Mohan H, Usha B. Safety, Dosage, and Regulatory Aspects in the Use of Phytochemicals. In: Medicinal Plants and Their Bioactives in Human Diseases. Springer; 2025. p. 207–33.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Ni Nyoman Gemini Sari, Raziv Ganesha, Ni Nyoman Linda Pradnya Ningsih
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
- Every manuscript submitted to must observe the policy and terms set by the Interdental Jurnal Kedokteran Gigi (IJKG)
- Publication rights to manuscript content published by the Interdental Jurnal Kedokteran Gigi (IJKG) is owned by the journal with the consent and approval of the author(s) concerned.
- Full texts of electronically published manuscripts can be accessed free of charge and used according to the license shown below.
