PROLIFERASI SEL ODONTOBLAS MENGGUNAKAN CAMPURAN MINERAL TRIOXIDE AGGREGATE (MTA) DENGAN BIODENTINE SECARA IN VITRO
Kata Kunci:
Pulp Capping, Calcium Hydroxide, MTA, Biodentine, Odontoblast Cell ProliferationAbstrak
Pulp capping is a treatment carried out by administering a bioactive material over the pulp tissue to stimulate the formation of reparative dentin. Materials that are often used in dentistry for pulp cap treatment are calcium hydroxide, mineral trioxide aggregate (MTA) and biodentine. This material can stimulate the formation of odontoblast-like cells. This research aims to determine the amount of proliferation in odontoblast cells by mixing mineral trioxide aggregate (MTA) with biodentine. The method of this research was a laboratory experiment consisting of two groups that were treated, namely calcium hydroxide (Ca(OH)2) as a control and a mixture of two materials, namely mineral trioxide aggregate (MTA) and biodentine. Before being treated, the odontoblast cells (cell line) as a sample were activated and subcultured first, then treated for seven days using the MTT assay method and the results of data analysis were read using a 595 nm wave ELISA reader. The results of the data analysis showed that there was a higher number of odontoblast cell proliferation between the mixture of MTA and biodentine compared to the control group, namely Ca(OH)2. Based on the results of the research carried out, it can be concluded that there is a difference in the amount of odontoblast cell proliferation between the mixture of MTA and biodentine and the control group, namely Ca(OH)2.
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