UJI DAYA HAMBAT EKSTRAK DAUN JARAK PAGAR (Jatropha curcas L.) TERHADAP PERTUMBUHAN BAKTERI Porphyromonas gingivalis SECARA IN VITRO
Kata Kunci:
Inhibition, Jatropha leaf extract (Jatropha curcas L.), Porphyromonas gingivalisAbstrak
Introduction: Chronic periodontitis is the disease most often found in periodontal tissue and generally occurs in adults caused by microorganisms found in dental plaque. The dominant microorganisms in chronic periodontitis are Porphyromonas gingivalis. Jatropha leaves (Jatropha curcas L.) contain active substances that have antibacterial. This content can inhibit the growth of the Porphyromonas gingivalis.
Methods: The method used in this research is a laboratory experimental research with the type of true experimental in vitro with the Post-Test Only Control Group Design. The treatments given was jatropha leaf extract (Jatropha curcas L.) with concentrations of 25%, 50%, and 100%, positive control with Metronidazole 500 mg and negative control
with Ethanol 96%.
Results: The results showed that the inhibitory power of Jatropha leaf extract (Jatropha curcas L.) at a concentration of 25% had an inhibition zone diameter of 10.87 ± 0.59 mm, concentration of 50% had an inhibition zone diameter of 13.27 ± 0.40 mm and 100% concentration had an inhibition zone diameter of 16.17 ± 0.25 mm for the growth of Porphyromonas gingivalis. Concentrations of 25%, 50%, and 100% have strong category inhibitory against Porphyromonas gingivalis bacteria. Based on the results of the Mann Whitney test, it showed that there were significant differences between treatment groups, with a p value <0.05 in all treatment groups. For 100% concentration (P: 0.000) and 50% concentration (P: 0.000).
Conclusion: The conclusion is that Jatropha leaf extract (Jatropha curcas L.) can inhibit the growth Porphyromonas gingivalis bacterial at concentrations of 25%, 50%, and 100% in vitro. Jatropha leaf extract (Jatropha curcas L.) 100% concentration is more effective in inhibition the growth Porphyromonas gingivalis compared to concentrations of 25% and 50%.
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