UJI DAYA HAMBAT EKSTRAK JINTAN HITAM (Nigella sativa) KONSENTRASI 50%, 75% DAN 100% TERHADAP PERTUMBUHAN BAKTERI Staphyloccocus aureus
Kata Kunci:
Staphylococcus aureus, dental root canal sterilization, inhibition, black cuminAbstrak
Introduction: This research is a laboratory research using Post Test Control Group Design. The samples used were Staphylococcus aureus bacteria which were divided into 5 groups, namely the group treated with black cumin extract with a concentration of 50%, 75% and 100%, the treatment group given ChKM as a positive control, and the group treated with aquadest as a negative control, with each 5 repetitions. Cumin extraction was carried out by maceration method using 96% ethanol solvent. Inhibition zone testing was carried out using the Kirby Bauer method. The inhibition produced at 50% extract concentration was 9.87 mm, 75% concentration was 14.24 mm and at 100% concentration was 17.19 mm. The higher the extract concentration, the greater the amount of antibacterial compounds released. Where antibacterial compounds identified are saponins, phenols, alkaloids, flavonoids, tannins. It can be concluded that the inhibition of black cumin extract (Nigella sativa) at concentrations of 50%, 75% and 100% can inhibit the growth of Staphylococcus aureus bacteria, with the most effective concentration being in the treatment group containing black cumin extract with a concentration of 100%.
Keywords: , , ,
Referensi
Andhiarto, Y., Andayani, R., & Ilmiyah, N. H. (2020). Uji Aktivitas Antibakteri Ekstrak Etanol 96% Daun Mimba (Azadirachta Indica A. Juss.) Dengan Metode Ekstraksi Perkolasi Terhadap Pertumbuhan Bakteri. Journal of Pharmacy Science and Technology, 2(1), 102-111.
Ayu, Z. P., & Pintadi, H. (2020). Daya Antibakteri Ekstrak Jintan Hitam dan Daun Sirih terhadap Staphylococcus aureus pada Plat Gigi Tiruan. Insisiva Dental Journal, 19-25.
Giovanni, A., Nahzi, M.Y.I. & Adhani, R., 2022, ‘Perbandingan Efektivitas Penggunaan Teknik Preparasi Saluran Akar Konvensional Dan Crown Terhadap Ekstruksi Debris Apikal’, Dentin 6(2).
Hasanah, D.A., Ayu, P.R. & Rahmawati, S., 2019, ‘Pengaruh Jintan Hitam (Nigella sativa) Terhadap Sindrom Metabolik Pada Wanita Menopause’, Medical Profession Journal of Lampung 9(2), 346-350.
Hidayati, S. & Subandi, L.Y., 2021, ‘Gambaran Pengetahuan Remaja Mengenai Karies Gigi Di Desa Petiken, Driyorejo, Gresik Tahun 2020’, Indonesian Journal of Health and Medical 1(3), 461- 469.
Islam, I.C., 2022, ‘Studi Efek Pemberian Ekstrak Minyak Jintan Hitam Terhadap Morfologi Anatomi Dan Gambaran Histologi Jarongan Saraf Pada Mencit Yang Diinduksi Etambutol: Study Of The Effect Of Black Cumin Oil Extract On Anatomi Morphology And Histological Description Of Ethambutol Induced Neural Tissue Damage In Mice’.
Kambaya, P.P., Jumiati, J. & Masyhudi, M., 2021, ;Uji Efek Antibakteri Ekstrak Etanol Buah Belimbing Wuluh (Averrhoa bilimbi L) sebagai Kandidat Bahan Medikamen Saluran Akar Gigi terhadap Pertumbuhan Staphylococcus aureus
secara In Vitro’, Mulawarman Dental Journal 1(1), 1-9.
Kartinawanti, A.T. & Asy'ari, A.K., 2021, ‘Penyakit Pulpa Dan Perawatan Saluran Akar Satu Kali Kunjungan’. Jurnal Ilmu Kedokteran Gigi 4(2), 64-72.
Makmun, A., Surdam, Z. & Gunawan, A.M., 2020, ‘Uji Efektivitas Ekstrak Jintan Hitam (Nigella sativa) terhadap Pertumbuhan Bakteri Staphylococcus aureus Pada Medium MHA (Mueller Hinton Agar)’, Window of Health: Jurnal Kesehatan, 001-009.
Permatasari, R. & Alifuddin, M.D., 2021, ‘Potensi regenerasi jaringan pulpa gigi pada perawatan endodontik’, M-Dental Education and Research Journal 1(2), 98- 110.
Permatasari, R., & Wulandari, D.S., 2022, ‘Potensi Antibakteri Triphala Sebagai Bahan Irigasi Saluran Akar Terhadap Bakteri Enterococcus faecalis’, Andalas Dental Journal 10(2), 84-91.
Rahmadani, D.D., 2021, ‘Hubungan Tingkat Pengetahuan Dengan Minat Pasien Melakukan Perawatan Saluran Akar di Masa Pandemi,’ (Doctoral dissertation, Poltekkes Kemenkes Yogyakarta).
Sinaga, A.B., Khasanah, F. & Suyatmi, D., 2021, ‘The Relationship of Knowledge About Dental Caries With The Motivation To Do Fillings in Housewives’, Journal of Oral Health Care 9 (1), 23-32.
Widariyani, W., 2019, ‘Gambaran Perilaku Pelihara Diri Kesehatan Gigi dan Mulut Terhadap Penyakit Pulpa Pada Pasien Usia 15-44 Tahun Di Puskesmas Berbah’, Jurnal Teknologi Keperawatan, 2(1), 1-37.
Yuanita, A.R., 2019, ‘Pengaruh Komunikasi Traupertik Terhadap Niat Pasien Menuntaskan Perawatan Saluran Akar (Studi Kasus di Poli Gigi Puskesmas Banjarejo Kota Madiun Tahun 2019)’, (Doctoral dissertation, Poltekkes Kemenkes Surabaya
