EFEKTIVITAS ANTIBAKTERI NANO KITOSAN SISIK IKAN NILA (Oreochromis niloticus) TAMBAK DANAU BATUR DITAMBAH NaOCl 1,5% DAN 2,5% TERHADAP BAKTERI Pseudomonas aeruginosa SEBAGAI BAHAN IRIGASI SALURAN AKAR GIGI
Kata Kunci:
Nano chitosan, Tilapia scales, NaOCl 1.5%, NaOCl 2.5%, Pseudomonas aeruginosAbstrak
Instroduction: Chitosan tilapia scales is one of the chitosans which has benefits as an antibacterial in dental root canal, the physical size of chitosan can be converted into smaller particle sizes in the form of nanoparticles. For the material of root canal irrigation as a gold standard is sodium hypochlorite 0.5% -5.25%. One of the bacteria found in root canal is Pseudomonas aeruginosa. The purpose of this study was to determine the effectiveness of nano chitosan tilapia scales plus 1.5% and 2.5% NaOCl in inhibiting Pseudomonas aeruginosa.
Methods: This research is a laboratory experimental study with the research design used is the Post Test Only Control Group Design antibacterial test Kirby Bauer. The sample used was the bacterium Pseudomonas aeruginosa which was divided into 4 groups: group 1 was the treatment group given nano chitosan of tilapia scales plus 5% NaOCl, group 2 was the treatment group given nano chitosan of tilapia scales plus 5% NaOCl, group 3 of the treatment group was given a positive control with Chlorhexidine and group 4 of the treatment group was given a negative control of aquadest with 6 repetitions for Result: The results of statistical tests using the Least Significant Difference (LSD) test showed that there were significant differences in the inhibition zone between nano chitosan of tilapia scale plus 1.5% and 2.5% NaOCl with a value of r <0.05. The average inhibition power of nano chitosan of tilapia scales plus 1.5% NaOCl was 21.33 mm and nano chitosan of tilapia scale plus 2.5% NaOCl was 24.83.
Conclusion: It can be concluded that nano chitosan of tilapia scale plus 1.5% NaOCl and nano chitosan of tilapia scale plus 2.5% NaOCl have very strong inhibition against Pseudomonas aeruginosa. Nano chitosan of tilapia scales plus 2.5% NaOCl is more effective in inhibiting Pseudomonas aeruginosa.
Referensi
Badan Penelitian dan Pengembangan Kesehatan Republik Indonesia. Laporan Hasil Riset Kesehatan Dasar (Riskesdas) Nasional., 2013, 10-7.
Kartinawanti AT, Asy'ari AK. Penyakit Pulpa Dan Perawatan Saluran Akar Satu Kali Kunjungan. JIKG (Jurnal Ilmu Kedokteran Gigi). 2021;4(2):64-72.
Grossman LI, Oliet S, Del Rio C E. Ilmu Endodontik Dalam Praktek, Edisi 11, EGC, Jakarta. 1995.
Hülsmann M, Hahn W. Complications during root canal irrigation–literature review and case reports. International endodontic journal. 2000 May 1;33(3).
Tanumihardja M. Larutan irigasi saluran akar. Journal of Dentomaxillofacial Science. 2010 Oct 30;9(2):108-15.
Yuanita T. The cleanliness differences of root canal walls after irrigated with East Java propolis extract and sodium hypoclorite solutions. Dental Journal (Majalah Kedokteran Gigi). 2017;50(1):6-10.
Zehnder M. Root canal irrigants. Journal of endodontics. 2006 May 1;32(5):389-398.
Tuncer AK. Effect of QMix 2in1 on sealer penetration into the dentinal tubules. Journal of endodontics. 2015 Feb 1;41(2):257-60
Brooks GF, Carroll KC, Butel JS, Morse. Mikrobiologi Kedokteran Jawetz, Melnick, & Adelberg. Ed. 25. Penerbit Buku Kedokteran EGC, Jakarta. 2013.
Azhar M, Efendi J, Sofyeni E, Lesi RF, Novalina S. Pengaruh konsentrasi NaOH dan KOH terhadap derajat deasetilasi kitin dari limbah kulit udang. Eksakta. 2010 Feb 2;1.
Sivakami MS, Gomathi T, Venkatesan J, Jeong HS, Kim SK, Sudha PN. Preparation and characterization of nano chitosan for treatment wastewaters. International journal of biological macromolecules. 2013 Jun 1;57:204-12.
Deviyanti S. Potensi Larutan Chitosan 0, 2% Sebagai Alternatif Bahan Irigasi Dalam Perawatan Saluran Akar Gigi. Jurnal Ilmiah dan Teknologi Kedokteran Gigi. 2018;14(1):6-10.
Adiana ID, Syafiar L. Penggunaan kitosan sebagai biomaterial di kedokteran gigi: use of chitosan as a biomaterial in dentistry. Dentika: Dental Journal. 2014 Dec 4;18(2):190-3.
Azizati Z. Pembuatan dan karakterisasi kitosan kulit udang galah. Walisongo Journal of Chemistry. 2019 Jun 13;2(1):10-6.
Khairunnisa S, Tandra TA, Sim M, Florenly F. Efektivitas antibakteri campuran nanokitosan 1% dengan berbagai konsentrasi ekstrak biji kelengkeng terhadap Staphylococcus aureus. Jurnal Ilmiah Kesehatan Sandi Husada. 2020;9(1):430-40.
Hudayni H. Sintesis kitosan dari limbah sisik ikan nila (oreochromis niloticus) sebagai adsorben logam cu2+’. Doctoral dissertation Universitas Mataram. 2018.
Sari DP, Nahzi, MY, Budiarti LY. Efektivitas daya hambat ekstrak umbi bawang dayak terstandarisasi fenol terhadap pertumbuhan Enterococcus faecalis. Dentin. 2019 Jan 29; 1(1).
Nesi MI, Sio S, Kia KW. Kualitas Mikrobiologi Dendeng Sapi yang di Curring dengan Ekstrak Rosella (Hibiscus sabdariffa. L). JAS. 2019 Jul 31;4(3):40-2.
Magani AK, Tallei TE, Kolondam BJ. Uji Antibakteri Nanopartikel Kitosan terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Escherichia coli. Jurnal Bios Logos. 2020 Jan 25;10(1):7-12.
Silalahi AM, Fadholah A, Artanti LO. Isolasi dan identifikasi kitin dan kitosan dari cangkang susuh kura (sulcospira testudinaria). Pharmaceutical Journal of Islamic Pharmacy. 2020 Mar 30;4(1):1-9
Darmawi I, Abidin T, Agusnar H, Gani BA. In Vitro Study of Irrigation solution of Chitosan Nanoparticles to Inhibit the Adhesion and Biofilm Formation of Enterococcus faecalis in the Root Canal. Research Journal of Pharmacy and Technology. 2022;15(6):2691-6.
Ratih DN, Sari NI, Santosa P, Kaswati NM. Time‐dependent effect of chitosan nanoparticles as final irrigation on the apical sealing ability and push‐out bond strength of root canal obturation. International Journal of Dentistry. 2020;2020(1):8887593.
