ACUTE TOXICITY TEST AND ALLERGY TEST OF GARLIC (Allium sativum L.) EXTRACT ON MICE (Mus Musculus)
Kata Kunci:
garlic (Allium sativum L.), allergy test, LD50, acute toxicityAbstrak
Background: Garlic is believed to have numerous benefits in addressing health issues, particularly in wound healing. Therefore, its safety needs to be assessed through acute toxicity and allergy tests. Acute toxicity testing is conducted to measure the degree of toxicity of a compound administered to test animals. Objective: This study aims to determine the toxicity and allergic effects of garlic extract (Allium sativum L.) on mice (Mus musculus). Method: This study employs an in vivo laboratory experimental design using the Post Test-Only Control Group Design. The acute toxicity test was conducted using the Organization for Economic Cooperation and Development (OECD) 425 method by calculating the lethal dose 50 (LD50) in mice (Mus musculus) with 25 subjects. Results: The acute toxicity test results showed that the LD50 value of garlic extract was 15 g/kg BW (mildly toxic) and did not show any allergic symptoms. The body weights of the mice before and after the treatment were analyzed using the OneWay ANOVA test. The analysis indicated no significant difference at a test level of 0.05 (p<0.05). Conclusion: Garlic extract (Allium sativum L.) does not cause allergies and does not affect toxicity levels at doses of 5000 mg/kg BW, 10,000 mg/kg BW, 15,000 mg/kg BW, and 20,000 mg/kg BW. However, it causes liver damage in mice at doses of 15,000 mg/kg BW and 20,000 mg/kg BW.
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