EFEKTIVITAS DAUN BIDARA ARAB (Ziziphus spina christ-L) TERHADAP DAYA HAMBAT BAKTERI Fusobacterium nucleatum
Kata Kunci:
Arabian bidara leaves (ziziphus spina christ-L), inhibitory power, antibacterial fusobacterium nucleatumAbstrak
Introduction: Fusobacterium nucleatum is a gram-negative anaerobic bacterium found predominantly in the oral cavity, playing a crucial role in the development of dental biofilms and contributing to periodontal diseases. Meanwhile, Arabian bidara leaves (Ziziphus spina-christ L.) are recognized for their potent antimicrobial properties due to the presence of flavonoids, alkaloids, steroids, saponins, and tannins, which exhibit significant inhibitory effects against various microorganisms. This study aims to assess the effectiveness of Arabian bidara leaf extract in inhibiting Fusobacterium nucleatum growth at different concentrations. Purpose: The primary objective of this research is to evaluate the inhibitory potency of Arabian bidara leaf extract (Ziziphus spina-christ L.) against Fusobacterium nucleatum bacteria. Methods: This experimental study utilized the Kirby Bauer method to investigate the antibacterial activity of Arabian bidara leaf extract in vitro. The research consisted of six repetitions, with four distinct treatment groups: 40% and 60% concentrations of the extract, a positive control using Tetracycline, and a negative control utilizing 96% Ethanol. Results: The results revealed that Arabian bidara leaf extract at a concentration of 60% exhibited an average inhibitory zone of 15,450 mm against Fusobacterium nucleatum. At 40% concentration, the average inhibitory zone measured 13,542 mm. The negative control consistently showed no significant reaction across all repetitions, while the positive control displayed resistance levels ranging from 19,200 mm to 19,800 mm. Statistical analysis using the Kruskall- Wallis test indicated significant differences among the treatment groups (p<0.05). Conclusion: In conclusion, Arabian bidara leaf extract demonstrated substantial inhibitory effects against Fusobacterium nucleatum bacteria, particularly at a concentration of 60%. These findings underscore the potential of Arabian bidara leaves as a natural antimicrobial agent in dental applications, particularly in combating bacterial species involved in dental biofilm formation and periodontal diseases.
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