Aphids Identification and Ability Test in Transmitting Zucchini Yellow Mosaic Virus (ZYMV)

  • Ni Putu Pandawani Faculty of Agriculture, University of Mahasaraswati Denpasar
  • I Ketut Widnyana Faculty of Agriculture, University of Mahasaraswati Denpasar
  • Putu Eka Pasmidi Ariati Faculty of Agriculture, University of Mahasaraswati Denpasar

Abstract

Zucchini (Cucurbita pepo L.) is a type of highland vegetable that has high economic value for farmers, but currently, farmers suffer losses due to the Zucchini Yellow Mosaic Virus (ZYMV) with a disease percentage of up to 89.80%. This study aims to identify the type of aphids as a ZYMV vector and determine its transmission potential. This research is an experimental study carried out in the laboratory and the field. Aphids were identified using the Blackman and Eastop identification keys. Serological tests to detect ZYMV are carried out using Enzyme-Linked Immunosorbent Assay (ELISA) with specific antiserum ZYMV. The results showed that there were three types of aphids as ZYMV vectors in the field, namely Aphis gossypii, Myzus persicae, and Aphis craccivora. Potential transmission of ZYMV from three different aphids, namely A. gossypii has the highest transmission ability of up to 70%, then M. persicae shows the transmission ability of 40% and A. craccivora shows the lowest transmission ability of 20%.

Published
2020-11-30